Deardorff T L, Throm R
Fishery Research Branch, Food and Drug Administration, Dauphin Island, Alabama 36528.
J Parasitol. 1988 Aug;74(4):600-3.
Sixty-four fish were blast-frozen to -35 C for 15 hr to determine the effects of commercial blast-freezing on the viability of third-stage larvae of Anisakis simplex encapsulated in the muscle and viscera of sockeye salmon (Oncorhynchus nerka) and canary rockfish (Sebastes pinniger). Parallel tests were conducted on larval nematodes in 16 whole (round) salmon, 16 dressed salmon (heads and viscera removed), and 32 whole (round) rockfish. After blast-freezing, 4 in-the-round salmon, 4 dressed salmon, and 8 in-the-round rockfish were examined at 1, 24, 48, and 72 hr. A total of 3,539 dead and 6 live larvae were collected from the fish tissues after standard enzymatic digestion. Salmon were infected with 1,245 of these larvae, and rockfish with 2,300. The 6 live worms, 2 from salmon and 4 from rockfish rounds, were recovered from muscle 1 hr after freezing; they were slightly motile and showed severe internal damage. No viable worms were found at or after 24 hr. The commercial blast-freezing process effectively killed larval nematodes in whole or dressed fish. Market-ready samples of previously blast-frozen silver salmon (O. kisutch) and chum salmon (O. keta) fillets and chum salmon steaks yielded no live worms, thereby confirming the efficacy of this process.
将64条鱼速冻至-35℃并保持15小时，以确定商业速冻对包裹在红大马哈鱼（Oncorhynchus nerka）和岩鱼（Sebastes pinniger）肌肉及内脏中的简单异尖线虫第三期幼虫活力的影响。对16条整条（圆形）大马哈鱼、16条去脏大马哈鱼（去除头部和内脏）以及32条整条（圆形）岩鱼体内的幼虫线虫进行了平行测试。速冻后，在1、24、48和72小时对4条整条大马哈鱼、4条去脏大马哈鱼和8条整条岩鱼进行检查。经标准酶消化后，从鱼组织中总共收集到3539条死幼虫和6条活幼虫。大马哈鱼感染了其中1245条幼虫，岩鱼感染了2300条。这6条活虫，2条来自大马哈鱼，4条来自整条岩鱼，在冷冻1小时后从肌肉中回收；它们活动轻微，内部有严重损伤。在24小时及之后未发现活虫。商业速冻过程有效地杀死了整条或去脏鱼体内的幼虫线虫。先前速冻的银大马哈鱼（O. kisutch）和狗鲑（O. keta）鱼片以及狗鲑鱼排的适销样品未检出活虫，从而证实了该过程的有效性。
