State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, Guangxi Key Laboratory of Sugarcane Biology, College of Agriculture, Guangxi University, Nanning, 530004 China.
College of Life Science and Technology, Guangxi University, Nanning, 530004 China.
Phytopathology. 2021 Dec;111(12):2303-2308. doi: 10.1094/PHYTO-03-21-0114-R. Epub 2021 Nov 22.
The biotrophic basidiomycetous fungus causing smut disease in sugarcane is characterized by a life cycle composed of a yeast-like nonpathogenic haploid basidiosporial stage outside the plant and filamentous pathogenic dikaryotic hyphae within the plant. Under field conditions, dikaryotic hyphae are formed after mating of two opposite mating-type strains. However, the mechanisms underlying genetic regulation of filamentation and its association with pathogenicity and development of teliospores are unclear. This study has focused on the characterization and genetic dissection of haploid filamentous mutants derived from T-DNA insertional mutagenesis. Our results support the existence of at least three genotypes among the six haploid filamentous mutants that differentially contribute to virulence and development of the whip and teliospore, providing a novel foundation for further investigation of the regulatory networks associated with pathogenicity and teliospore development in .
引起甘蔗黑穗病的生物亲和担子菌真菌的特点是生活史由植物外的酵母样非致病性单倍体担子孢子阶段和植物内的丝状致病性双核菌丝组成。在田间条件下，双核菌丝是由两个相反交配型菌株交配后形成的。然而，丝状形成的遗传调控机制及其与致病性和冬孢子发育的关系尚不清楚。本研究集中于从小麦纹枯病菌 T-DNA 插入突变体库中筛选获得的单倍体丝状突变体的鉴定和遗传分析。我们的结果支持在这 6 个单倍体丝状突变体中至少存在 3 种基因型，它们对鞭毛和冬孢子的毒力和发育有不同的影响，为进一步研究与致病性和冬孢子发育相关的调控网络提供了新的基础。
