Zhu Guining, Deng Yizhen, Cai Enping, Yan Meixin, Cui Guobing, Wang Zhiqiang, Zou Chengwu, Zhang Bin, Xi Pinggen, Chang Changqing, Chen Baoshan, Jiang Zide
Guangdong Province Key Laboratory of Microbial Signals and Disease Control, State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, Department of Plant Pathology, South China Agricultural University, Guangzhou, China.
Guangxi Key Laboratory of Biology for Crop Diseases and Insect Pests, Plant Protection Research Institute, Guangxi Academy of Agricultural Sciences, Nanning, China.
Front Microbiol. 2019 Sep 10;10:2115. doi: 10.3389/fmicb.2019.02115. eCollection 2019.
The sugarcane smut fungus is bipolar and produces sporidia of two different mating types. During infection, haploid cells of opposite mating types can fuse to form dikaryotic hyphae that can colonize plant tissue. Mating and filamentation are therefore essential for pathogenesis. In this study, we obtained one T-DNA insertion mutant disrupted in the gene encoding the pheromone response factor (Prf1), hereinafter named , of , via -mediated transformation (ATMT) mutagenesis. Targeted deletion of resulted in mutants with phenotypes similar to the T-DNA insertion mutant, including failure to mate with a compatible wild-type partner strain and being non-pathogenic on its host sugarcane. qRT-PCR analyses showed that was essential for the transcription of pheromone-responsive mating type genes of the locus. These results show that is involved in mating and pathogenicity and plays a key role in pheromone signaling and filamentous growth in .
甘蔗黑粉菌是双极性的，可产生两种不同交配型的担孢子。在感染过程中，不同交配型的单倍体细胞可融合形成能在植物组织中定殖的双核菌丝。因此，交配和菌丝形成对于致病过程至关重要。在本研究中，我们通过农杆菌介导的转化（ATMT）诱变获得了一个T-DNA插入突变体，该突变体在编码信息素反应因子（Prf1）的基因中发生了破坏，以下简称为Prf1。对Prf1进行靶向缺失导致突变体出现与T-DNA插入突变体相似的表型，包括无法与相容的野生型伙伴菌株交配以及对其寄主甘蔗无致病性。qRT-PCR分析表明，Prf1对于该位点信息素反应性交配型基因的转录至关重要。这些结果表明，Prf1参与交配和致病性，并在甘蔗黑粉菌的信息素信号传导和丝状生长中起关键作用。
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