Key Laboratory of Marine Drugs, Ministry of Education, School of Medicine and Pharmacy and Shandong Provincial Key laboratory of Glycoscience and Glycoengineering, Ocean University of China, 5 Yushan Road, Qingdao 266003, China.
Laboratory for Marine Drugs and Bioproducts, Pilot National Laboratory for Marine Science and Technology (Qingdao), Wenhai Road, Qingdao 266237, China.
Glycobiology. 2021 Sep 9;31(8):931-946. doi: 10.1093/glycob/cwab037.
Glycan microarrays have played important roles in detection and specificity assignment of glycan recognition by proteins. However, the size and diversity of glycan libraries in current microarray systems are small compared to estimated glycomes, and these may lead to missed detection or incomplete assignment. For microarray construction, covalent and noncovalent immobilization are the two types of methods used, but a direct comparison of results from the two platforms is required. Here we develop a chemical strategy to prepare lipid-linked probes from both naturally derived aldehyde-terminating and synthetic amino-terminating glycans that addresses the two aspects: expansion of sequence-defined glycan libraries and comparison of the two platforms. We demonstrate the specific recognition by plant and mammalian lectins, carbohydrate-binding modules and antibodies and the overall similarities from the two platforms. Our results provide new knowledge on unique glycan-binding specificities for the immune receptor Dectin-1 toward β-glucans and the interaction of rotavirus P[19] adhesive protein with mucin O-glycan cores.
糖芯片在糖蛋白对糖识别的检测和特异性分配中发挥了重要作用。然而，与估计的糖组相比，当前微阵列系统中的糖库的大小和多样性较小，这可能导致漏检或不完全分配。对于微阵列构建，共价和非共价固定化是两种使用的方法，但需要对来自两个平台的结果进行直接比较。在这里，我们开发了一种从天然衍生的醛端和合成的氨基端聚糖制备脂连接探针的化学策略，该策略解决了两个方面的问题：扩展序列定义的聚糖文库和比较两个平台。我们展示了植物和哺乳动物凝集素、碳水化合物结合模块和抗体的特异性识别以及两个平台的整体相似性。我们的结果为免疫受体 Dectin-1 对β-葡聚糖的独特糖结合特异性以及轮状病毒 P[19]粘附蛋白与粘蛋白 O-聚糖核心的相互作用提供了新知识。
