聚糖微阵列筛选平台。
Microbe-focused glycan array screening platform.
机构信息
Department of Biomolecular Systems, Max Planck Institute of Colloids and Interfaces, 14424 Potsdam, Germany.
Institute of Chemistry and Biochemistry, Freie Universität Berlin, 14195 Berlin, Germany.
出版信息
Proc Natl Acad Sci U S A. 2019 Feb 5;116(6):1958-1967. doi: 10.1073/pnas.1800853116. Epub 2019 Jan 22.
Interactions between glycans and glycan binding proteins are essential for numerous processes in all kingdoms of life. Glycan microarrays are an excellent tool to examine protein-glycan interactions. Here, we present a microbe-focused glycan microarray platform based on oligosaccharides obtained by chemical synthesis. Glycans were generated by combining different carbohydrate synthesis approaches including automated glycan assembly, solution-phase synthesis, and chemoenzymatic methods. The current library of more than 300 glycans is as diverse as the mammalian glycan array from the Consortium for Functional Glycomics and, due to its microbial focus, highly complementary. This glycan platform is essential for the characterization of various classes of glycan binding proteins. Applications of this glycan array platform are highlighted by the characterization of innate immune receptors and bacterial virulence factors as well as the analysis of human humoral immunity to pathogenic glycans.
糖缀合物与糖结合蛋白之间的相互作用是所有生命领域中许多过程所必需的。糖微阵列是研究蛋白-糖缀合物相互作用的极好工具。在这里，我们提出了一种基于化学合成获得的寡糖的微生物聚焦糖微阵列平台。糖通过结合不同的碳水化合物合成方法来产生，包括自动糖基化组装、溶液相合成和化学酶法方法。目前，这个拥有 300 多种糖的文库与功能糖组学联盟的哺乳动物糖阵列一样多样化，并且由于其微生物聚焦，具有高度互补性。这个糖基平台对于各种糖结合蛋白的特性分析是必不可少的。该糖基平台的应用通过对先天免疫受体和细菌毒力因子的特性分析以及对人类针对致病糖的体液免疫的分析得到了强调。
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