炎症因子浸润诱导慢性前列腺炎阴茎损伤。

Infiltration of inflammatory factors induced penile damage in chronic prostatitis.

机构信息

Department of Urology, Shanghai Putuo District People's Hospital, Tongji University, Shanghai, China.

Department of Urology, Shanghai Tenth People's Hospital, School of Medicine, Tongji University, Shanghai, China.

出版信息

Andrologia. 2021 Sep;53(8):e14113. doi: 10.1111/and.14113. Epub 2021 May 12.

DOI:10.1111/and.14113

PMID:33979463

Abstract

The present study aimed to investigate the mechanism of penile damages in experimental autoimmune prostatitis (EAP) rat models to reveal the potential pathological mechanism of the relationship between CP and penile damages. Sprague-Dawley (SD) rats were administered with different concentrations of prostate tissue homogenate supernatant (PTHS) by multipoint subcutaneous injection to establish EAP models. IHC staining was done to assess the expression of inflammatory cytokines in prostate tissues and the corpus cavernosum of penis. Masson and Tunel staining was conducted to observe the fibrosis and apoptosis in the corpus cavernosum. Finally, the functional changes of corpus cavernosum were assessed by WB and IHC staining. The results revealed that EAP rats with different prostatitis severity were successfully established by PTHS. The expression of IL-1β, IL-6 and TNF-α in prostate tissues increased with the concentration of PTHS. The results of Masson and Tunel staining indicated fibrosis and apoptosis gradually aggravated in corpus cavernosum among different subgroups. The function of cavernosum impaired by prostatitis from WB and IHC results and positively with the severity. In conclusion, there existed the infiltration of inflammatory factors and impaired function in the corpus cavernosum of EAP rats' penis and positively correlated with the severity of prostatitis.

摘要

本研究旨在探讨实验性自身免疫性前列腺炎（EAP）大鼠模型中阴茎损伤的机制，以揭示 CP 与阴茎损伤之间关系的潜在病理机制。通过多点皮下注射不同浓度的前列腺组织匀浆上清液（PTHS），建立 EAP 模型。免疫组化染色评估前列腺组织和阴茎海绵体中炎症细胞因子的表达。Masson 和 Tunel 染色观察海绵体的纤维化和凋亡。最后，通过 WB 和 IHC 染色评估海绵体的功能变化。结果表明，成功地通过 PTHS 建立了不同前列腺炎严重程度的 EAP 大鼠模型。随着 PTHS 浓度的增加，前列腺组织中 IL-1β、IL-6 和 TNF-α 的表达增加。Masson 和 Tunel 染色结果表明，不同亚组海绵体中的纤维化和凋亡逐渐加重。从 WB 和 IHC 结果来看，海绵体的功能因前列腺炎而受损，且与前列腺炎的严重程度呈正相关。总之，EAP 大鼠阴茎海绵体存在炎症因子浸润和功能障碍，且与前列腺炎的严重程度呈正相关。