Green D P
Department of Pharmacology, University of Otago Medical School, Dunedin, New Zealand.
J Reprod Fertil. 1988 May;83(1):377-87. doi: 10.1530/jrf.0.0830377.
A method has been developed for establishing from electron micrographs the radius of curvature of rabbit and guinea-pig sperm nuclei in head-to-tail section (the major axis), and of guinea-pig spermatozoa with intact acrosomes in transverse section (the minor axis). Radii of curvature of head-to-tail sections lie, for the most part, between 7 and 100 microns in both species. Guinea-pig sperm nuclei in transverse section show a range of radii between 3 and 10 microns, and approximate, therefore, caps of prolate spheroids. The method is not applicable to rat and mouse spermatozoa but Nomarski light microscopy suggests that these nuclei are straight in the longitudinal direction. The distribution of the radii of curvature in rabbit sperm nuclei is similar to that of the radii of curvature of penetration slits through the zona pellucida of the rabbit egg. It is suggested that longitudinal curvature of sperm heads is correlated with any curvature of the penetration slit.
已开发出一种方法，可从电子显微照片确定兔和豚鼠精子核在头尾截面（长轴）的曲率半径，以及豚鼠具有完整顶体的精子在横向截面（短轴）的曲率半径。在这两个物种中，头尾截面的曲率半径大部分在7至100微米之间。豚鼠精子核在横向截面的曲率半径范围为3至10微米，因此近似于长椭球体的帽。该方法不适用于大鼠和小鼠精子，但诺马斯基相差显微镜显示这些核在纵向是直的。兔精子核曲率半径的分布与穿过兔卵透明带的穿透缝曲率半径的分布相似。有人提出，精子头部的纵向曲率与穿透缝的任何曲率相关。
