Toshimori K
Biol Reprod. 1982 Apr;26(3):475-81. doi: 10.1095/biolreprod26.3.475.
The mouse sperm head was examined at the site of fertilization in vivo by 200KV electron microscopy. The spermatozoa penetrating through the zona pellucida had already completed the acrosome reaction and discarded the acrosome. Therefore, the inner acrosomal membrane over-lying the rostral region was directly exposed to surrounding substances. In such spermatozoa, the inner acrosomal membrane was continuous with the outer acrosomal membrane at the posterior boundary of the equatorial segment, while the outer acrosomal membrane was continuous with the plasma membrane at the anterior boundary of the equatorial segment. The equatorial segment, with such a continuity among the membranes, was still intact in the perivitelline space. These morphological characteristics of the mouse sperm head are discussed in relation to the mode of fertilization.
通过200KV电子显微镜在体内受精部位对小鼠精子头部进行了检查。穿透透明带的精子已经完成顶体反应并丢弃了顶体。因此,覆盖在头部区域的顶体内膜直接暴露于周围物质。在这类精子中,顶体内膜在赤道段的后边界与顶体外膜连续,而顶体外膜在赤道段的前边界与质膜连续。在卵周隙中,具有这种膜间连续性的赤道段仍然完好无损。本文结合受精方式对小鼠精子头部的这些形态学特征进行了讨论。