ICMR-National AIDS Research Institute, Pune, Maharashtra, India. Correspondence to: Dr Madhuri Thakar, Head and Scientist-F, Department of Immunology and Serology, ICMR, National AIDS Research Institute, Plot No. 73, G-Block, MIDC, Bhosari, Pune-411026, Maharashtra, India.
ICMR-National AIDS Research Institute, Pune, Maharashtra, India.
Indian Pediatr. 2021 May 15;58(5):424-429.
To determine the reference ranges of various lymphocyte subsets in healthy Indian children.
Descriptive cross-sectional study.
Four centers in India representing four geographical regions.
1104 children from neonatal age to 18 years of age.
One time measurement of absolute count and percentages of different lymphocyte subsets i.e. T lymphocytes (CD3+T, CD4+T, CD8+T cells), B lymphocytes (CD19+B cells) and Natural Killer lymphocytes (CD15/16+NK cells) in whole blood using multicolor flow cytometry.
The absolute cell counts of various lymphocytes were found to increase from newborn to 10 months of age, followed by gradual decline until 18 years; however, the proportion of immune cells remained largely similar. Gender did not have a significant impact on the reference ranges, whereas counts were found to vary as per the geographical locations.
These reference ranges will be useful to monitor and predict the immune status in pediatric population. The variation in region wise ranges could be confirmed by testing more number of samples in the specific age groups.
确定健康印度儿童各种淋巴细胞亚群的参考范围。
描述性横断面研究。
印度的四个中心，代表四个地理区域。
1104 名年龄从新生儿到 18 岁的儿童。
使用多色流式细胞术一次性测量全血中不同淋巴细胞亚群（T 淋巴细胞[CD3+T、CD4+T、CD8+T 细胞]、B 淋巴细胞[CD19+B 细胞]和自然杀伤淋巴细胞[CD15/16+NK 细胞]的绝对计数和百分比。
发现各种淋巴细胞的绝对细胞计数从新生儿到 10 个月龄增加，然后逐渐下降至 18 岁；然而，免疫细胞的比例基本保持相似。性别对参考范围没有显著影响，而计数则因地理位置而异。
这些参考范围将有助于监测和预测儿科人群的免疫状况。可以通过在特定年龄组中测试更多数量的样本来确认按地区划分的范围的变化。
