印度儿童不同淋巴细胞亚群的参考范围：一项多中心研究。

Reference Ranges of Different Lymphocyte Subsets in Indian Children: A Multi-Centric Study.

机构信息

ICMR-National AIDS Research Institute, Pune, Maharashtra, India. Correspondence to: Dr Madhuri Thakar, Head and Scientist-F, Department of Immunology and Serology, ICMR, National AIDS Research Institute, Plot No. 73, G-Block, MIDC, Bhosari, Pune-411026, Maharashtra, India.

ICMR-National AIDS Research Institute, Pune, Maharashtra, India.

出版信息

Indian Pediatr. 2021 May 15;58(5):424-429.

PMID:33980728

Abstract

OBJECTIVE

To determine the reference ranges of various lymphocyte subsets in healthy Indian children.

DESIGN

Descriptive cross-sectional study.

SETTING

Four centers in India representing four geographical regions.

PARTICIPANTS

1104 children from neonatal age to 18 years of age.

MEASUREMENT

One time measurement of absolute count and percentages of different lymphocyte subsets i.e. T lymphocytes (CD3+T, CD4+T, CD8+T cells), B lymphocytes (CD19+B cells) and Natural Killer lymphocytes (CD15/16+NK cells) in whole blood using multicolor flow cytometry.

RESULTS

The absolute cell counts of various lymphocytes were found to increase from newborn to 10 months of age, followed by gradual decline until 18 years; however, the proportion of immune cells remained largely similar. Gender did not have a significant impact on the reference ranges, whereas counts were found to vary as per the geographical locations.

CONCLUSIONS

These reference ranges will be useful to monitor and predict the immune status in pediatric population. The variation in region wise ranges could be confirmed by testing more number of samples in the specific age groups.

摘要