Zhan Wen-Li, Yang Xiao-Han, Guo Hao, Zhong Zhi-Cheng, DU Qian-Yi, Zhang Chang-Bin, Huang Bin-Mei, Liu Hong, Luo Ming-Yong
Medical Genetics Center, Guangdong Women and Children's Hospital, Guangzhou 511442, China.
Zhongguo Dang Dai Er Ke Za Zhi. 2019 Feb;21(2):180-183. doi: 10.7499/j.issn.1008-8830.2019.02.015.
To study the distribution of peripheral blood lymphocyte subsets in healthy children aged 0-6 years.
A total of 826 healthy Han children aged 0-6 years were recruited. According to their age, the children were divided into four groups: newborn, infant, toddler and preschool. Their peripheral blood samples were collected to measure the percentages of lymphocyte subsets by flow cytometry.
There were significant differences in the percentages of CD3 T cells, CD3CD4 T cells and CD3CD19 B cells and the CD4/CD8 ratio between boys and girls (P<0.05). The girls had a lower percentage of CD3CD19 B cells, higher percentages of CD3 T cells and CD3CD4 T cells and a higher CD4/CD8 ratio than the boys. The newborn group had the highest percentages of CD3 T cells and CD3CD4 T cells and the highest CD4/CD8 ratio (P<0.05). The percentage of CD3CD4 T cells and the CD4/CD8 ratio gradually decreased with age and the preschool group had the lowest values (P<0.05). The newborn group had the lowest percentages of CD3CD19 B cells and CD3CD16CD56 NK cells (P<0.05). The percentage of CD3CD16CD56 NK cells gradually increased with age and the preschool group had the highest percentage (P<0.05). The percentage of CD3CD19 B cells reached the peak in the toddler period and then decreased with age (P<0.05). The preschool group had the highest percentage of CD3CD8 T cells (P<0.05). The variation trend of distribution of lymphocyte subsets in boys from different age groups was consistent with that in children from different age groups. For girls, the newborn group had the highest percentage of CD3CD4 T cells and CD4/CD8 ratio (P<0.05).
The distribution of peripheral blood lymphocyte subsets in healthy children is significantly different across ages and sexes. Therefore, the reference values should be established according to age and sex.
研究0 - 6岁健康儿童外周血淋巴细胞亚群的分布情况。
共招募826名0 - 6岁健康汉族儿童。根据年龄将儿童分为四组：新生儿组、婴儿组、幼儿组和学龄前儿童组。采集他们的外周血样本，通过流式细胞术检测淋巴细胞亚群的百分比。
男孩和女孩的CD3⁺T细胞、CD3⁺CD4⁺T细胞和CD3⁺CD19⁺B细胞百分比以及CD4⁺/CD8⁺比值存在显著差异（P<0.05）。女孩的CD3⁺CD19⁺B细胞百分比低于男孩，CD3⁺T细胞、CD3⁺CD4⁺T细胞百分比及CD4⁺/CD8⁺比值高于男孩。新生儿组的CD3⁺T细胞、CD3⁺CD4⁺T细胞百分比及CD4⁺/CD8⁺比值最高（P<0.05）。CD3⁺CD4⁺T细胞百分比及CD4⁺/CD8⁺比值随年龄逐渐降低，学龄前儿童组最低（P<0.05）。新生儿组的CD3⁺CD19⁺B细胞和CD3⁺CD16⁺CD56⁺NK细胞百分比最低（P<0.05）。CD3⁺CD16⁺CD56⁺NK细胞百分比随年龄逐渐升高，学龄前儿童组最高（P<0.05）。CD3⁺CD19⁺B细胞百分比在幼儿期达到峰值，随后随年龄降低（P<0.05）。学龄前儿童组CD3⁺CD8⁺T细胞百分比最高（P<0.05）。不同年龄组男孩淋巴细胞亚群分布的变化趋势与不同年龄组儿童一致。对于女孩，新生儿组的CD3⁺CD4⁺T细胞百分比及CD4⁺/CD8⁺比值最高（P<0.05）。
健康儿童外周血淋巴细胞亚群的分布在年龄和性别上存在显著差异。因此，应根据年龄和性别建立参考值。
