Li Huan, Chen Juanjuan, Hu Yuanhui, Cai Xin, Zhang Pingan
Department of Clinical Laboratory, Renmin Hospital of Wuhan University, Wuhan, China.
Front Pediatr. 2021 Apr 26;9:619899. doi: 10.3389/fped.2021.619899. eCollection 2021.
To analyze the serum complement C1q levels in children with sepsis, and explore the suggestive effect of serum C1q levels on the condition of children with sepsis. The clinical and laboratory data of children with sepsis ( = 95) and healthy children ( = 71) in Renmin Hospital of Wuhan University from January 2019 to October 2019 were collected, and each index of the two groups was compared. Then we divided children with sepsis into three subgroups based on the Pediatric Critical Illness Score (PCIS): non-critical group, critical group, and extremely critical group. The serum C1q and PCT levels of the three subgroups were analyzed, and the correlation analysis was carried out between the levels of serum C1q and PCT levels as well as PCIS among children with sepsis. Finally, we analyzed the serum C1q levels of septic children infected by different pathogens. The serum C1q levels of children with sepsis were significantly higher than those of healthy children (median 198.4 vs. 186.2 mg/L, < 0.001). In the analysis of subgroups, the serum C1q levels of non-critical group, critical group, and extremely critical group septic children were 182.80 (166.75, 195.85) mg/L, 219.90 (209.10, 246.40) mg/L and 249.95 (239.10, 272.25) mg/L, respectively, which were correlated with the severity of the disease. At the same time, we also found that serum C1q in children with sepsis was positively correlated with PCT levels ( = 0.5982, < 0.001), and negatively correlated with PCIS score ( = -0.6607, < 0.001). The serum C1q levels of septic children with bacterial infections, mycoplasma infections, viral infections, and co-infection were higher than those of the control group ( < 0.05). The serum levels of C1q in children with sepsis were increased and related to the severity of sepsis, suggesting that C1q may be involved in the occurrence and development of sepsis, which had reference value for the preliminary diagnosis and severity classification of sepsis.
分析脓毒症患儿血清补体C1q水平，探讨血清C1q水平对脓毒症患儿病情的提示作用。收集武汉大学人民医院2019年1月至2019年10月脓毒症患儿（n = 95）和健康儿童（n = 71）的临床及实验室资料，比较两组各项指标。然后根据儿童危重病评分（PCIS）将脓毒症患儿分为三个亚组：非危重组、危重组和极危重组。分析三个亚组的血清C1q和PCT水平，并对脓毒症患儿血清C1q水平与PCT水平以及PCIS进行相关性分析。最后分析不同病原体感染的脓毒症患儿的血清C1q水平。脓毒症患儿血清C1q水平显著高于健康儿童（中位数198.4 vs. 186.2 mg/L，P < 0.001）。亚组分析中，非危重组、危重组和极危重组脓毒症患儿血清C1q水平分别为182.80（166.75，195.85）mg/L、219.90（209.10，246.40）mg/L和249.95（239.10，272.25）mg/L，与疾病严重程度相关。同时，还发现脓毒症患儿血清C1q与PCT水平呈正相关（r = 0.5982，P < 0.001），与PCIS评分呈负相关（r = -0.6607，P < 0.001）。细菌感染、支原体感染、病毒感染及混合感染的脓毒症患儿血清C1q水平均高于对照组（P < 0.05）。脓毒症患儿血清C1q水平升高且与脓毒症严重程度相关，提示C1q可能参与脓毒症的发生发展，对脓毒症的初步诊断及严重程度分级有参考价值。
