Topalidou Irini
Department of Biochemistry, University of Washington, Seattle, WA 98195.
MicroPubl Biol. 2021 May 9;2021. doi: 10.17912/micropub.biology.000393.
The FLP-1 neuropeptide is involved in locomotion, mechanosensation, and reproduction. Strain RB2126 is reported to contain the mutation, a prospective null allele. Here we report that strain RB2126, in addition to , also contains two additional background mutations. One of the mutations gives the animals a constitutive dauer phenotype (Daf-c), and complementation testing confirms that it is in the Daf-c gene The second mutation is isolated based on the fact that it partially suppresses the elevated body curvature of mutants, but it remains uncloned. The updated genotype of RB2126 strain is
FLP-1神经肽参与运动、机械感觉和繁殖过程。据报道,RB2126菌株含有该突变,这是一个预期的无效等位基因。在此我们报告,RB2126菌株除了含有该突变外,还包含另外两个背景突变。其中一个突变使动物呈现组成型滞育表型(Daf-c),互补试验证实该突变位于Daf-c基因中。第二个突变是基于它部分抑制了突变体升高的身体弯曲度这一事实分离得到的,但尚未克隆。RB2126菌株的更新基因型为