Su Jie, Wang Ting, Yan Mei-Qiu, Yu Jing-Jing, Xu Jian-Bo, Zhuge Rui-Cheng, Yu Huan-Huan, Chen Su-Hong, Lyu Gui-Yuan
Zhejiang Chinese Medical University Hangzhou 310053, China.
Zhejiang University of Technology Hangzhou 310014, China.
Zhongguo Zhong Yao Za Zhi. 2021 Apr;46(7):1658-1666. doi: 10.19540/j.cnki.cjcmm.20201027.403.
Dendrobii officinalis, with a definite effect of nourishing Yin and clearing heat, has been a folk habit for drinking after being mixed with water. Because its superfine powder has the advantages of high dissolution and convenient drinking, we observed the effect of D. officinalis superfine powder on metabolic hypertension model rats and its possible mechanism in this experiment, which can be used as a reference for its clinical application for hypertension. The overeating greasy-induced metabolic hypertension model was established with high-fat, high-sugar and high-purine diet. These rats were orally administered with 400 mg·kg(-1) and 200 mg·kg(-1) of D. officinalis superfine powder for 20 consecutive weeks. During this period, blood pressure, blood lipid, blood glucose, insulin and other related indexes of glucose and lipid metabolism were monitored; the levels of lipopolysaccharide(LPS), C-reactive protein(CRP), interleukin 6(IL-6) and other inflammatory mediators were measured; the levels of nitric oxide(NO) and endothelin-1(ET-1) were detected, and the histomorphological and ultrastructural changes of aorta were observed. In addition, the expression of LPS/TLR4 pathway-related molecules in aorta was determined. The results showed that long-term administration of D. officinalis superfine powder significantly reduced the levels of systolic blood pressure(SBP), diastolic blood pressure(DBP) and mean arterial pressure(MBP) in metabolic hypertension model rats, decreased the levels of total cholesterol(TC), triglyceride(TG), low density lipoprotein cholesterol(LDL-c), glucose(Glu), and insulin(INS) levels in blood, increased the contents of high density lipoprotein cholesterol(HDL-c),decreased the LPS, CRP, IL-6 and ET-1 levels in blood and increased NO content. Furthermore, it improved the abnormality of aortic histomorphology and endothelial ultrastructure, and inhibited the protein expression of TLR4, myeloid differentiation factor(MyD88), IL-6, interleukin-1 β(IL-1β), and tumor necrosis factor-α(TNF-α) as well as mRNA expression of TNF-α and IL-1β in aorta. In conclusion, D. officinalis superfine powder may improve the abnormal function and structure of blood vessels by inhibiting the activation of LPS/TLR4 pathway, thus playing a role against metabolic hypertension.
铁皮石斛具有明确的滋阴清热功效，民间素有将其泡水饮用的习惯。因其超微粉具有溶出度高、服用方便等优点，本实验观察了铁皮石斛超微粉对代谢性高血压模型大鼠的作用及其可能机制，可为其临床治疗高血压提供参考。采用高脂、高糖、高嘌呤饮食建立饮食积滞型代谢性高血压模型。将大鼠连续20周分别灌胃给予400 mg·kg⁻¹和200 mg·kg⁻¹的铁皮石斛超微粉。在此期间，监测血压、血脂、血糖、胰岛素等糖脂代谢相关指标；检测脂多糖（LPS）、C反应蛋白（CRP）、白细胞介素6（IL-6）等炎症介质水平；检测一氧化氮（NO）和内皮素-1（ET-1）水平，并观察主动脉的组织形态学和超微结构变化。此外，测定主动脉中LPS/TLR4通路相关分子的表达。结果表明，长期给予铁皮石斛超微粉可显著降低代谢性高血压模型大鼠的收缩压（SBP）、舒张压（DBP）和平均动脉压（MBP）水平，降低血液中总胆固醇（TC）、甘油三酯（TG）、低密度脂蛋白胆固醇（LDL-c）、血糖（Glu）和胰岛素（INS）水平，升高高密度脂蛋白胆固醇（HDL-c）含量，降低血液中LPS、CRP、IL-6和ET-1水平，升高NO含量。此外，还改善了主动脉组织形态学和内皮超微结构的异常，抑制了主动脉中TLR4、髓样分化因子（MyD88）、IL-6、白细胞介素-1β（IL-1β）和肿瘤坏死因子-α（TNF-α）的蛋白表达以及TNF-α和IL-1β的mRNA表达。综上所述，铁皮石斛超微粉可能通过抑制LPS/TLR4通路的激活改善血管功能和结构异常，从而发挥抗代谢性高血压的作用。
