Programa de Pós-Graduação em Pediatria, Universidade Federal de Ciências da Saúde de Porto Alegre, Porto Alegre, Rio Grande do Sul, Brazil.
Laboratório de Fisiologia Comportamental e Metabólica, Universidade Federal de Ciências da Saúde de Porto Alegre, Porto Alegre, Rio Grande do Sul, Brazil.
Zebrafish. 2021 Jun;18(3):184-189. doi: 10.1089/zeb.2020.1910. Epub 2021 May 12.
Melatonin is a hormone related to circadian rhythms and has potential clinical applications. Our objectives were to verify the effect of melatonin on the liver of zebrafish exposed to fructose and evaluate the expression of appetite-related genes (leptin, ghrelin, and melanocortin receptor 4 [MC4R]). Animals were divided into three groups: control (CG, = 25), fructose (FG, = 25), and fructose+melatonin (FMG, = 25). The study was carried out in 8 weeks. FG and FMG were exposed to 2% fructose and FMG treated with 1 μM of melatonin. Histological liver studies and gene expression analyses of Leptin, Ghrelin, and MC4R (liver and intestines) were performed. FG developed hepatic steatosis, which did not occur with CG and FMG. Genetic expression of hepatic leptin and MC4R did not show significant difference among the groups. Animals exposed to fructose (FG) presented an increased expression of intestinal leptin compared to those administered with melatonin. Animals exposed to fructose gained weight and developed an important hepatic steatosis, but melatonin reduced significantly the hepatic damage. Intestinal leptin showed increased expression in the group exposed to fructose.
褪黑素是一种与昼夜节律相关的激素，具有潜在的临床应用价值。我们的目的是验证褪黑素对果糖暴露的斑马鱼肝脏的影响，并评估与食欲相关的基因（瘦素、胃饥饿素和黑素皮质素受体 4 [MC4R]）的表达。动物分为三组：对照组（CG，n=25）、果糖组（FG，n=25）和果糖+褪黑素组（FMG，n=25）。研究持续了 8 周。FG 和 FMG 暴露于 2%的果糖中，FMG 用 1μM 的褪黑素处理。进行了肝脏组织学研究和 Leptin、Ghrelin 和 MC4R（肝脏和肠道）的基因表达分析。FG 出现了肝脂肪变性，但 CG 和 FMG 没有。肝脏瘦素和 MC4R 的基因表达在各组之间没有显著差异。与给予褪黑素的动物相比，暴露于果糖的动物肠道瘦素的表达增加。暴露于果糖的动物体重增加，并出现严重的肝脂肪变性，但褪黑素显著减轻了肝损伤。暴露于果糖的组中，肠道瘦素的表达增加。
