SKF-96365 对卵清蛋白诱导的小鼠变应性鼻炎的治疗作用。
Therapeutic effects of SKF-96365 on murine allergic rhinitis induced by OVA.
机构信息
Department of Otolaryngology-Head and Neck Surgery, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai, China.
Otolaryngological Institute, Shanghai Jiao Tong University, Shanghai, China.
出版信息
Int J Immunopathol Pharmacol. 2021 Jan-Dec;35:20587384211015054. doi: 10.1177/20587384211015054.
INTRODUCTION
SKF-96365 is regarded as an inhibitor of receptor-mediated calcium ion (Ca) entry. The current study aimed to explore the effects of SKF-96365 on murine allergic rhinitis (AR).
METHODS
Intranasal SKF-96365 administration was performed on OVA induced murine AR. Serum and nasal lavage fluid (NLF) from mice were harvested to assay IgE and inflammatory cytokines using ELISA method. Inflammatory cells were counted and analyzed in NLF. Nasal mucosa tissues were collected from mice and used for HE staining, immunohistochemistry (IHC) staining, and real-time PCR detection.
RESULTS
SKF-96365 had therapeutic effects on murine AR manifesting attenuation of sneezing, nasal rubbing, IgE, inflammatory cytokines, inflammatory cells, TRPC6 immunolabeling, and TRPC6, STIM1 and Orai1 mRNA levels in AR mice.
CONCLUSION
SKF-96365 could effectively alleviate the symptoms of murine AR. SKF-96365 could suppress TRPC6, STIM1, and Orai1 activities, leading to the downregulation of inflammatory cytokines and inflammatory cells in murine AR.
简介
SKF-96365 被认为是一种受体介导热离子（Ca）内流的抑制剂。本研究旨在探讨 SKF-96365 对小鼠变应性鼻炎（AR）的影响。
方法
通过鼻内给予 SKF-96365 对卵清蛋白（OVA）诱导的小鼠 AR 进行干预。采集血清和鼻洗液（NLF），采用 ELISA 法检测 IgE 和炎症细胞因子。对 NLF 中的炎症细胞进行计数和分析。收集小鼠鼻黏膜组织，进行 HE 染色、免疫组织化学（IHC）染色和实时 PCR 检测。
结果
SKF-96365 对小鼠 AR 具有治疗作用，表现为打喷嚏、鼻擦减少，IgE、炎症细胞因子、炎症细胞、TRPC6 免疫标记和 AR 小鼠的 TRPC6、STIM1 和 Orai1 mRNA 水平降低。
结论
SKF-96365 可有效缓解小鼠 AR 的症状。SKF-96365 可抑制 TRPC6、STIM1 和 Orai1 的活性，从而下调小鼠 AR 中的炎症细胞因子和炎症细胞。
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