Zhang Weitian, Tang Ru, Ba Guangyi, Li Mingxian, Lin Hai
Department of Otolaryngology-Head and Neck Surgery, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai, PR China.
Otolaryngological Institute, Shanghai Jiao Tong University, Shanghai, PR China.
World Allergy Organ J. 2020 Oct 22;13(10):100473. doi: 10.1016/j.waojou.2020.100473. eCollection 2020 Oct.
Allergic rhinitis (AR) is a type I hypersensitivity mediated by IgE in the nose. Thioredoxin-interacting protein (TXNIP) plays a pivotal role in the process of producing reactive oxygen species (ROS). Resveratrol is a TXNIP inhibitor. Nonetheless, its role and mechanism in AR are still undetermined. The present study aimed to explore the effect and mechanism of resveratrol on an ovalbumin (OVA) induced mouse model of AR.
AR murine model was established using OVA and administrated intranasally with resveratrol or -acetylcysteine (NAC). Hematoxylin and eosin (HE) stain was used for evaluating eosinophils. Immunohistochemistry (IHC) staining and real-time PCR were employed to evaluate immunolabeling and mRNA expression of TXNIP in nasal mucosas of mice. Malondialdehyde (MDA) level and superoxide dismutase (SOD) activity in nasal tissue homogenates were measured using MDA and SOD Assay Kit. Concentrations of OVA-specific IgE and histamines in serum, and OVA-specific IgE, PGD2, LTC4, ECP, IL-4, IL-5, IL-6, IL-33 and TNF-α in nasal lavage fluid (NLF) were assayed by ELISA. studies, western blotting, real-time PCR, ELISA, ROS detecting dye DCFH-DA, MDA, and SOD Assay Kit were performed to evaluate the effects and mechanisms of OVA, resveratrol or NAC on spleen mononuclear cells.
We found significant alternations of sneezing, nasal rubbing, inflammatory cytokines, eosinophil numbers, TXNIP, MDA, and SOD levels in resveratrol or NAC treated mice compared with untreated AR mice. In cultured spleen mononuclear cells, TXNIP, MDA, SOD, ROS and inflammatory cytokines levels were altered by OVA but reversed by resveratrol or NAC.
Resveratrol could effectively alleviate murine AR by inhibiting TXNIP-oxidative stress pathway.
变应性鼻炎（AR）是由鼻内IgE介导的I型超敏反应。硫氧还蛋白相互作用蛋白（TXNIP）在活性氧（ROS）产生过程中起关键作用。白藜芦醇是一种TXNIP抑制剂。然而，其在AR中的作用和机制仍未明确。本研究旨在探讨白藜芦醇对卵清蛋白（OVA）诱导的AR小鼠模型的影响及机制。
用OVA建立AR小鼠模型，并经鼻给予白藜芦醇或N-乙酰半胱氨酸（NAC）。采用苏木精-伊红（HE）染色评估嗜酸性粒细胞。采用免疫组织化学（IHC）染色和实时PCR评估小鼠鼻黏膜中TXNIP的免疫标记和mRNA表达。使用丙二醛（MDA）和超氧化物歧化酶（SOD）检测试剂盒测量鼻组织匀浆中的MDA水平和SOD活性。通过ELISA检测血清中OVA特异性IgE和组胺的浓度，以及鼻灌洗液（NLF）中OVA特异性IgE、PGD2、LTC4、ECP、IL-4、IL-5、IL-6、IL-33和TNF-α的浓度。进行研究、蛋白质印迹法、实时PCR、ELISA、ROS检测染料DCFH-DA、MDA和SOD检测试剂盒以评估OVA、白藜芦醇或NAC对脾单核细胞的影响及机制。
我们发现，与未治疗的AR小鼠相比，白藜芦醇或NAC治疗的小鼠在打喷嚏、鼻摩擦、炎性细胞因子、嗜酸性粒细胞数量、TXNIP、MDA和SOD水平上有显著变化。在培养的脾单核细胞中，TXNIP、MDA、SOD、ROS和炎性细胞因子水平受OVA影响，但被白藜芦醇或NAC逆转。
白藜芦醇可通过抑制TXNIP-氧化应激途径有效减轻小鼠AR。
