Effects of organic acid, Enterococcus faecalis strain EC-12 and sugar cane extract in feed against enterotoxigenic Escherichia coli-induced diarrhea in pigs.

Diarrhea can lead to mortality and delayed growth in pigs and is a major economic loss in the pig industry. In this study, we evaluated non-antimicrobial materials that can prevent diarrhea to infection by enterotoxigenic Escherichia coli (ETEC) in weaned pigs and investigated biological changes. We confirmed the efficacy of fumaric acid, lactic acid, Enterococcus faecalis strain EC-12 (EC12) and sugar cane extract (SCE) in inhibiting diarrhea and investigated the biological changes by analyzing gut microbiota and plasma metabolites. Administration of EC12 (0.1%, w/w) and SCE (1.0%, w/w) groups had reduced score of diarrhea. Furthermore, the combination of EC12 and SCE was effective at reducing the fecal score of diarrhea even at low concentrations. Administration of either EC12 or SCE greatly reduced the relative abundance of Enterobacteriaceae in pigs. EC12 and SCE were most effective in suppressing ETEC-induced diarrhea in weaned pigs. Furthermore, we were able to identify biological changes in pigs when EC12 and SCE were administered to pigs. These feeds may have prevented infection by ETEC in weaned pigs and may improve pig productivity and reduce the use of antimicrobial agents.