人类粗球孢子菌病中 IgE 识别的枝孢菌、雷氏普罗威登斯菌和美洲粗球孢子菌的独特抗原模式。
Distinct Pattern of Paracoccidioides lutzii, P. restrepiensis and P. americana Antigens Recognized by IgE in Human Paracoccidioidomycosis.
机构信息
State University of Londrina, Londrina, PR, Brazil.
Departamento de Ciências Patológicas, CCB, Universidade Estadual de Londrina, Campus Universitário, Londrina, PR, 86051-970, Brazil.
出版信息
Curr Microbiol. 2021 Jul;78(7):2608-2614. doi: 10.1007/s00284-021-02508-7. Epub 2021 May 13.
Paracoccidioidomycosis (PCM) is caused by the fungi Paracoccidioides spp. The main antigens recognized by IgE are known for P. brasiliensis species complex, but not for P. lutzii. Current research investigated the major P. lutzii (LDR2) antigens recognized by IgE, in comparison to P. restrepiensis and P. americana (former P. brasiliensis species complex), besides IgG recognition. Cell-free antigens (CFA) from P. lutzii (LDR2), P. restrepiensis (B339) and P. americana (LDR3) were analyzed by ELISA and immunoblotting (IB) by detecting specific IgG and IgE from sera from patients with PCM presumable by P. brasiliensis species complex (n = 24). Additionally, somatic antigen (SA) was analyzed by IB. P. lutzii (LDR2) antigens showed significantly lower reactivity than P. restrepiensis (B339) and P. americana (LDR3) in ELISA for both IgE and IgG (p < 0.05). The IgE-IB pattern was different between P. lutzii (LDR2) and the other species, regarding components with ~30 kDa and ~70 kDa in CFA and a ~200 kDa in SA. P. lutzii (LDR2) present at least three antigens recognized by IgE which mainly differ from P. restrepiensis (B339) and, to a lesser extent, from P. americana (LDR3). Current research evidenced for the first time the major P. lutzii (LDR2) antigens recognized by IgE.
球孢子菌病（PCM）是由球腔菌属（Paracoccidioides spp.）引起的。已知与巴西副球孢子菌复合体相关的 IgE 主要识别抗原，但与卢氏球孢子菌（P. lutzii）无关。目前的研究调查了与 P. restrepiensis 和 P. americana（前巴西副球孢子菌复合体）相比，IgE 识别的主要卢氏球孢子菌（LDR2）抗原，以及 IgG 识别。通过酶联免疫吸附试验（ELISA）和免疫印迹（IB）分析卢氏球孢子菌（LDR2）、P. restrepiensis（B339）和 P. americana（LDR3）的无细胞抗原（CFA），并检测疑似巴西副球孢子菌复合体感染的 PCM 患者血清中的特异性 IgG 和 IgE（n = 24）。此外，还通过 IB 分析了体细胞抗原（SA）。与 P. restrepiensis（B339）和 P. americana（LDR3）相比，卢氏球孢子菌（LDR2）抗原在 ELISA 中检测 IgE 和 IgG 时的反应性显著降低（p < 0.05）。在 CFA 中，IgE-IB 模式在卢氏球孢子菌（LDR2）和其他物种之间存在差异，与约 30 kDa 和约 70 kDa 的成分以及 SA 中的约 200 kDa 成分有关。卢氏球孢子菌（LDR2）至少存在三种被 IgE 识别的抗原，这些抗原主要与 P. restrepiensis（B339）不同，与 P. americana（LDR3）的差异较小。目前的研究首次证明了卢氏球孢子菌（LDR2）的主要 IgE 识别抗原。
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