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中国利用纳米孔测序技术获得的 基因组序列资源。

Genome Sequence Resource of from China Using Nanopore Sequencing Technology.

机构信息

Key Laboratory of Green Prevention and Control of Tropical Plant Diseases and Pests, Ministry of Education, College of Plant Protection, Hainan University, Haikou 570228, China.

Fujian University Key Laboratory for Plant-Microbe Interaction, College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou, 350002, China.

出版信息

Plant Dis. 2021 Dec;105(12):4141-4145. doi: 10.1094/PDIS-11-20-2327-A. Epub 2021 Dec 3.

Abstract

is a destructive oomycete pathogen of taro (), which causes taro leaf blight. To date, only one highly fragmented Illumina short-read-based genome assembly is available for this species. To address this problem, we sequenced strain Lyd2019 from China using Oxford Nanopore Technologies long-read sequencing and Illumina short-read sequencing. We generated a 92.51-Mb genome assembly consisting of 105 contigs with an N of 1.70 Mb and a maximum length of 4.17 Mb. In the genome assembly, we identified 52.78% repeats and 18,322 protein-coding genes, of which 12,782 genes were annotated. We also identified 191 candidate RXLR effectors and 1 candidate crinkling and necrosis effector. The updated near-chromosome genome assembly and annotation resources will provide a better understanding of the infection mechanisms of .

摘要

是芋头()的一种具有破坏性的卵菌病原体,可引起芋头叶斑病。迄今为止,该物种仅提供了一个高度碎片化的基于 Illumina 短读测序的基因组组装。为了解决这个问题,我们使用 Oxford Nanopore Technologies 长读测序和 Illumina 短读测序对来自中国的菌株 Lyd2019 进行了测序。我们生成了一个由 105 个 contigs 组成的 92.51-Mb 基因组组装,N 值为 1.70 Mb,最大长度为 4.17 Mb。在基因组组装中,我们鉴定了 52.78%的重复序列和 18322 个蛋白质编码基因,其中 12782 个基因被注释。我们还鉴定了 191 个候选 RXLR 效应子和 1 个卷曲和坏死效应子。更新的近染色体基因组组装和注释资源将更好地了解 的感染机制。

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