Department of Biomedical Engineering, Dr. N.G.P. Institute of Technology, Coimbatore 641048, India.
Department of Chemistry, Vel Tech Multi Tech Engineering College, Chennai 600062, India.
Int J Biol Macromol. 2021 Jul 31;183:1106-1118. doi: 10.1016/j.ijbiomac.2021.05.039. Epub 2021 May 10.
A dual layered herbal biopolymeric patch (biopatch) with enhanced wound healing efficiency and skin mimicking functions was fabricated for skin-tissue regenerative applications. In this study, hoof keratin (KE) extracted from biological waste and gelatin (GE) was employed for KE-GE biosheet fabrication using a simple casting method. Further, the top layer of the fabricated KE-GE biosheet was coated with bioactive Matricaria recutita (Chamomile flower) extract (CH) with gelatin through an electrospraying method. The optimized dual layered herbal biopatch (KE-GE/GE-CH) exhibits strong physiochemical (FTIR, XRD TG-DTA), mechanical (tensile strength) and biological (in vitro and in vivo) studies. Moreover, the morphology (SEM) of soft mimetic biopatch possesses excellent cell-material interaction and cell proliferation which accelerates the wound healing process. Biopatch demonstrates a proven degradation profile with good swelling features to achieve more than 80% herbal drug release in 96 h. Antimicrobial properties also reveal the potential activity of biopatch against bacterial microbes. In addition, in vitro cell viability using NIH 3T3 fibroblast cell lines and in vivo investigations revealed that the biopatch is non-cytotoxic, increases collagen deposition and shows rapid reepithelialization at the wound site as a potential wound dressing. We anticipated that the biological hoof keratin and bioactive herbal extract coated biopatch could serve as a desirable wound dressing candidate to suit various skin tissue regenerative applications.
一种具有增强的伤口愈合效率和皮肤模拟功能的双层草药生物聚合物贴片（生物贴片）被制造用于皮肤组织再生应用。在这项研究中，从生物废物中提取的蹄角蛋白（KE）和明胶（GE）用于使用简单的铸造方法制造 KE-GE 生物片。此外，通过静电喷涂方法，在制备的 KE-GE 生物片的顶层涂覆有含有明胶的生物活性洋甘菊（洋甘菊花）提取物（CH）。优化的双层草药生物贴（KE-GE/GE-CH）具有强大的物理化学（FTIR、XRD TG-DTA）、机械（拉伸强度）和生物学（体外和体内）研究。此外，柔软仿生生物贴的形态（SEM）具有出色的细胞-材料相互作用和细胞增殖，从而加速了伤口愈合过程。生物贴显示出经过验证的降解曲线，具有良好的溶胀特性，可在 96 小时内释放超过 80%的草药药物。抗菌性能也揭示了生物贴对细菌微生物的潜在活性。此外，使用 NIH 3T3 成纤维细胞系进行的体外细胞活力和体内研究表明，生物贴是非细胞毒性的，可增加胶原蛋白沉积，并在伤口部位迅速再上皮化，作为一种潜在的伤口敷料。我们预计，生物蹄角蛋白和含有生物活性草药提取物的涂层生物贴片可以作为一种理想的伤口敷料候选物，适用于各种皮肤组织再生应用。
