Università degli Studi di Milano, Facoltà di Medicina e Chirurgia, Sezione di Medicina Legale e delle Assicurazioni, Dipartimento di Scienze Biomediche per la Salute, Via Luigi Mangiagalli 37, 20133 Milano, Italy.
Università degli Studi di Milano, Facoltà di Medicina e Chirurgia, Sezione di Medicina Legale e delle Assicurazioni, Dipartimento di Scienze Biomediche per la Salute, Via Luigi Mangiagalli 37, 20133 Milano, Italy.
Leg Med (Tokyo). 2021 Sep;52:101905. doi: 10.1016/j.legalmed.2021.101905. Epub 2021 May 7.
The microscopic examination of a hanging cutaneous furrow, stained with Goldner's Masson trichrome staining, highlighted an abnormal dyeing inversion affinity of the connective tissue - red instead of green - located in the region of maximal cutaneous compression and exsiccation. To identify if this different stainability could be considered as an intrinsic characteristic of all biological tissues compressed and exsiccated, we have assessed different cadaveric skin samples that underwent traumatic detrimental phenomena that can produce such effects. We collected skin fragments from 24 corpses deceased because of gunshot injuries, electrocution, hanging, and heat-induced lesions, sampling the areas directly involved, as well as skin specimens to use as a control. The slides were stained with hematoxylin and eosin and two different protocols of Goldner's Masson trichrome staining (one homemade and one commercial kit). The inversion of the staining affinity of the connective tissue was observed in 83% of the cutaneous samples, using both the Goldner's Masson trichrome staining protocols. This phenomenon was not observed in any of the control cases. Therefore, the inversion of the staining affinity of the connective tissue dyed with Goldner's Masson trichrome staining may represent a histomorphological aspect that must be expected when the skin has been affected by specific detrimental modalities able of producing compression and exsiccation.
对悬挂皮肤皱襞的显微镜检查，用 Goldner 氏 Masson 三色染色染色，突出显示了结缔组织的异常染色反转亲和力 - 红色而不是绿色 - 位于最大皮肤压迫和干燥区域。为了确定这种不同的染色是否可以被认为是所有被压缩和干燥的生物组织的固有特征，我们评估了不同的尸体皮肤样本，这些样本经历了可能产生这种效果的创伤性有害现象。我们从因枪击伤、触电、悬挂和热诱导损伤而死亡的 24 具尸体中收集了皮肤碎片，采集了直接受累的区域，以及用作对照的皮肤标本。载玻片用苏木精和伊红以及两种不同的 Goldner 氏 Masson 三色染色方案（一种自制的和一种商业试剂盒）进行染色。在使用两种 Goldner 氏 Masson 三色染色方案时，观察到 83%的皮肤样本中结缔组织的染色亲和力发生反转。在任何对照病例中都没有观察到这种现象。因此，Goldner 氏 Masson 三色染色染色的结缔组织染色亲和力的反转可能代表一种组织形态学方面，当皮肤受到能够产生压迫和干燥的特定有害方式影响时，必须预期到这种现象。
