Pospíšil Jakub, Wiebusch Gerd, Fliegel Karel, Klíma Miloš, Huser Thomas
Opt Express. 2021 Apr 12;29(8):11833-11844. doi: 10.1364/OE.420592.
Current super-resolution structured illumination microscopes (SR-SIM) utilize relatively expensive electro-optic components and free-space optics, resulting in large setups. Moreover, high power laser sources are required to compensate for the losses associated with generating the illumination pattern by diffractive optics. Here, we present a highly compact and flexible 2D SR-SIM microscope based on all-fiber optic components (fiberSIM). Fiber-splitters deliver the laser light to the sample resulting in the interference illumination pattern. A microelectromechanical systems (MEMS) based fiber switch performs rapid pattern rotation. The pattern phase shift is achieved by the spatial displacement of one arm of the fiber interferometer using a piezoelectric crystal. Compared with existing methods, fiberSIM is highly compact and significantly reduces the SR-SIM component cost while achieving comparable results, thus providing a route to making SR-SIM technology accessible to even more laboratories in the life sciences.
当前的超分辨率结构光照明显微镜（SR-SIM）使用相对昂贵的电光组件和自由空间光学器件，导致设备体积庞大。此外，需要高功率激光源来补偿与通过衍射光学器件生成照明图案相关的损耗。在此，我们展示了一种基于全光纤组件的高度紧凑且灵活的二维SR-SIM显微镜（fiberSIM）。光纤分束器将激光传输到样品上，从而产生干涉照明图案。基于微机电系统（MEMS）的光纤开关执行快速图案旋转。通过使用压电晶体使光纤干涉仪的一个臂发生空间位移来实现图案相移。与现有方法相比，fiberSIM高度紧凑，在实现可比结果的同时显著降低了SR-SIM组件成本，从而为使更多生命科学实验室能够使用SR-SIM技术提供了一条途径。
