Xu Fanghui, Zhang Jiachen, Ding Dongdong, Liu Wenjie, Zheng Chi, Zhou Sijia, Chen Youhua, Kuang Cuifang
Opt Express. 2022 Apr 11;30(8):13238-13251. doi: 10.1364/OE.454982.
Structured illumination microscopy (SIM), a super-resolution technology, has a wide range of applications in life sciences. In this study, we present an electro-optic high-speed phase-shift super-resolution microscopy imaging system including 2D SIM, total internal reflection fluorescence-SIM, and 3D SIM modes. This system uses galvanometers and an electro-optic modulator to flexibly and quickly control the phase and direction of structured illumination patterns. Moreover, its design consists of precise timing for improved acquisition speed and software architecture for real-time reconstruction. The highest acquisition rate achieved was 151 frames/s, while the highest real-time super-resolution reconstruction frame rate achieved was over 25 frames/s.
结构光照明显微镜（SIM）作为一种超分辨率技术，在生命科学领域有着广泛的应用。在本研究中，我们展示了一种电光高速相移超分辨率显微镜成像系统，该系统包括二维SIM、全内反射荧光-SIM和三维SIM模式。该系统使用振镜和电光调制器来灵活快速地控制结构光照图案的相位和方向。此外，其设计包括用于提高采集速度的精确计时和用于实时重建的软件架构。实现的最高采集速率为151帧/秒，而实现的最高实时超分辨率重建帧率超过25帧/秒。
