Department of Laboratory Diagnostics, Medical University of Lodz, Poland.
Department of Internal Medicine, Medical University of Lodz, Poland.
J Biol Regul Homeost Agents. 2021 Mar-Apr;35(2):485-494. doi: 10.23812/20-689-A.
Adhesion molecules, as such, play essential roles in T-cell transendothelial extravasation during inflammation. A better understanding of the mechanisms underlying this process may be of value in the management of asthma. The present study employed Magnetic-Activated Cell Sorting (MACS) to isolate human CD8 T lymphocytes from peripheral blood of asthma patients and controls. The cells were flow cytometrically assessed to evaluate surface expression of an adhesion molecule, L-selectin (CD62L) on the surface of CD8FoxP3 T cell subsets and its response to inflammatory cytokines. We showed that CD8CD28TCRαβCD62LFoxP3 T cells were deficient in blood of some asthma patients but abundant in others. After co-stimulation of CD8 T cells with anti-CD3/CD28 in combination with IL-2 and IL-10 or TGF-β, the frequencies of CD8CD28TCRα/βCD62L T cells in the group of patients were lower than at baseline. Our data indicate that L-selectin expression is regulated by inflammatory cytokines. Overall, these data reveal that asthma phenotypes may be further stratified into micro subtypes with distinct cellular and molecular characteristics, supporting the concept of asthma endotypes.
黏附分子在炎症过程中 T 细胞穿过血管内皮迁移过程中发挥重要作用。更好地理解这一过程的机制可能有助于哮喘的治疗。本研究采用磁激活细胞分选（MACS）技术从哮喘患者和健康对照者的外周血中分离 CD8 T 淋巴细胞。通过流式细胞术评估细胞表面黏附分子 L-选择素（CD62L）在 CD8 FoxP3 T 细胞亚群表面的表达及其对炎症细胞因子的反应。结果显示，一些哮喘患者的血液中 CD8CD28TCRαβCD62LFoxP3 T 细胞数量减少，而另一些哮喘患者血液中该细胞数量增加。在 CD8 T 细胞与抗 CD3/CD28 联合 IL-2 和 IL-10 或 TGF-β 共刺激后，与基线相比，患者组 CD8CD28TCRα/βCD62L T 细胞的频率降低。我们的数据表明，L-选择素的表达受炎症细胞因子的调节。总之，这些数据表明哮喘表型可能进一步分为具有不同细胞和分子特征的微亚型，支持哮喘内型的概念。
