Institut Jean-Pierre Bourgin, UMR 1318, INRAE, AgroParisTech, Université Paris-Saclay, Versailles, France.
Université Paris-Sud, Université Paris-Saclay, Orsay, France.
Nat Commun. 2021 May 13;12(1):2787. doi: 10.1038/s41467-021-22995-3.
Transgenes that are stably expressed in plant genomes over many generations could be assumed to behave epigenetically the same as endogenous genes. Here, we report that whereas the histone H3K9me2 demethylase IBM1, but not the histone H3K4me3 demethylase JMJ14, counteracts DNA methylation of Arabidopsis endogenous genes, JMJ14, but not IBM1, counteracts DNA methylation of expressed transgenes. Additionally, JMJ14-mediated specific attenuation of transgene DNA methylation enhances the production of aberrant RNAs that readily induce systemic post-transcriptional transgene silencing (PTGS). Thus, the JMJ14 chromatin modifying complex maintains expressed transgenes in a probationary state of susceptibility to PTGS, suggesting that the host plant genome does not immediately accept expressed transgenes as being epigenetically the same as endogenous genes.
在许多代中稳定表达于植物基因组的转基因可以假定在表观遗传学上与内源性基因表现相同。在这里，我们报告说，组蛋白 H3K9me2 去甲基化酶 IBM1 而非组蛋白 H3K4me3 去甲基化酶 JMJ14 拮抗拟南芥内源性基因的 DNA 甲基化，而 JMJ14 而非 IBM1 拮抗表达的转基因的 DNA 甲基化。此外，JMJ14 介导的转基因 DNA 甲基化的特异性衰减增强了易诱导系统转录后转基因沉默（PTGS）的异常 RNA 的产生。因此，JMJ14 染色质修饰复合物使表达的转基因处于易受 PTGS 影响的试验状态，表明宿主植物基因组不会立即将表达的转基因视为在表观遗传学上与内源性基因相同。
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