鸡接种新城疫病毒后的胸腺转录组分析及宿主小 RNA 对 NDV 复制的影响。
Thymic transcriptome analysis after Newcastle disease virus inoculation in chickens and the influence of host small RNAs on NDV replication.
机构信息
Henan University of Animal Husbandry and Economy, No. 2 Yingcai Street, Huiji District, Zhengzhou, 450044, China.
South China Agricultural University, Guangzhou, 510000, China.
出版信息
Sci Rep. 2021 May 13;11(1):10270. doi: 10.1038/s41598-021-89464-1.
Newcastle disease (ND), caused by virulent Newcastle disease virus (NDV), is a contagious viral disease affecting various birds and poultry worldwide. In this project, differentially expressed (DE) circRNAs, miRNAs and mRNAs were identified by high-throughput RNA sequencing (RNA-Seq) in chicken thymus at 24, 48, 72 or 96 h post LaSota NDV vaccine injection versus pre-inoculation group. The vital terms or pathways enriched by vaccine-influenced genes were tested through KEGG and GO analysis. DE genes implicated in innate immunity were preliminarily screened out through GO, InnateDB and Reactome Pathway databases. The interaction networks of DE innate immune genes were established by STRING website. Considering the high expression of gga-miR-6631-5p across all the four time points, DE circRNAs or mRNAs with the possibility to bind to gga-miR-6631-5p were screened out. Among DE genes that had the probability to interact with gga-miR-6631-5p, 7 genes were found to be related to innate immunity. Furthermore, gga-miR-6631-5p promoted LaSota NDV replication by targeting insulin induced gene 1 (INSIG1) in DF-1 chicken fibroblast cells. Taken together, our data provided the comprehensive information about molecular responses to NDV LaSota vaccine in Chinese Partridge Shank Chickens and elucidated the vital roles of gga-miR-6631-5p/INSIG1 axis in LaSota NDV replication.
新城疫（ND）是由强毒新城疫病毒（NDV）引起的一种传染性病毒性疾病，影响全球各种鸟类和家禽。在本项目中，通过高通量 RNA 测序（RNA-Seq）在鸡胸腺中鉴定了 24、48、72 或 96 h 接种 LaSota NDV 疫苗后与接种前组相比的差异表达（DE）circRNAs、miRNAs 和 mRNAs。通过 KEGG 和 GO 分析测试了疫苗影响基因富集的重要术语或途径。通过 GO、InnateDB 和 Reactome 通路数据库初步筛选出与先天免疫相关的 DE 基因。通过 STRING 网站建立 DE 先天免疫基因的互作网络。考虑到 gga-miR-6631-5p 在所有四个时间点的高表达，筛选出可能与 gga-miR-6631-5p 结合的 DE circRNAs 或 mRNAs。在有可能与 gga-miR-6631-5p 相互作用的 DE 基因中，发现有 7 个基因与先天免疫有关。此外，gga-miR-6631-5p 通过靶向鸡成纤维细胞（DF-1）中的胰岛素诱导基因 1（INSIG1）促进 LaSota NDV 复制。总之，我们的数据提供了关于中国鹧鸪胫鸡对 NDV LaSota 疫苗反应的综合信息，并阐明了 gga-miR-6631-5p/INSIG1 轴在 LaSota NDV 复制中的重要作用。
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