Department of Radiology, Hadassah Medical Center, The Faculty of Medicine, Hebrew University of Jerusalem, 9112001, Jerusalem, Israel.
Department of Psychiatry and Psychotherapie I (Weissenau), Ulm University, ZfP Suedwuerttemberg, Ravensburg, Germany.
Sci Rep. 2021 May 13;11(1):10211. doi: 10.1038/s41598-021-89534-4.
Direct and real-time monitoring of cerebral metabolism exploiting the drastic increase in sensitivity of hyperpolarized C-labeled metabolites holds the potential to report on neural activity via in-cell metabolic indicators. Here, we followed the metabolic consequences of curbing action potential generation and ATP-synthase in rat cerebrum slices, induced by tetrodotoxin and oligomycin, respectively. The results suggest that pyruvate dehydrogenase (PDH) activity in the cerebrum is 4.4-fold higher when neuronal firing is unperturbed. The PDH activity was 7.4-fold reduced in the presence of oligomycin, and served as a pharmacological control for testing the ability to determine changes to PDH activity in viable cerebrum slices. These findings may open a path towards utilization of PDH activity, observed by magnetic resonance of hyperpolarized C-labeled pyruvate, as a reporter of neural activity.
利用超极化 C 标记代谢物灵敏度的大幅提高，直接实时监测脑代谢，有可能通过细胞内代谢指标报告神经活动。在这里，我们分别通过河豚毒素和寡霉素抑制大鼠脑切片动作电位产生和 ATP 合酶，来研究代谢后果。结果表明，神经元放电不受干扰时，脑内丙酮酸脱氢酶（PDH）活性高 4.4 倍。寡霉素存在时，PDH 活性降低了 7.4 倍，可作为药理学对照，用于测试检测活脑切片中 PDH 活性变化的能力。这些发现可能为利用磁共振超极化 C 标记丙酮酸观察到的 PDH 活性作为神经活动的报告器开辟了一条道路。
