Klimowicz A
Pomeranian Medical Academy Clinic of Dermatology, Szczecin, Poland.
Methods Find Exp Clin Pharmacol. 1988 May;10(5):331-6.
Analysis of sulphadimethoxine in plasma from patients treated with this drug was performed with four chemical methods: Rieder's modification of the Bratton-Marshall technique and author's modification of the Morris technique, which are supposed to measure "potentially active fraction of sulphonamides", i.e., mainly unchanged sulphonamide; the Bratton-Marshall method, which enables the determination of "free sulphonamide", i.e., the sum of unchanged sulphonamide and glucuronide conjugate; and high performance liquid chromatography which made possible the determination of unchanged sulphadimethoxine. The spectrophotometric methods based upon Bratton-Marshall reaction gave very few higher results as compared with liquid chromatography. The differences between spectrophotometric and chromatographic methods were small; therefore, all four methods can be used for determination of sulphonamide concentrations in plasma during monitored treatment with these drugs.
里德对布拉顿 - 马歇尔技术的改进方法以及作者对莫里斯技术的改进方法，这两种方法旨在测定“磺胺类药物的潜在活性部分”，即主要是未变化的磺胺；布拉顿 - 马歇尔方法，该方法能够测定“游离磺胺”，即未变化的磺胺和葡萄糖醛酸共轭物的总和；以及高效液相色谱法，该方法可用于测定未变化的磺胺二甲氧嘧啶。与液相色谱法相比，基于布拉顿 - 马歇尔反应的分光光度法得出的较高结果很少。分光光度法和色谱法之间的差异很小；因此，在这些药物的监测治疗期间，所有这四种方法均可用于测定血浆中磺胺类药物的浓度。
