Zhang Linna, Cao Huimei, Tao Hong, Yang Jijuan, Gong Wei, Hu Qikuan
Department of Physiology, Ningxia Medical University, Yinchuan, Ningxia Hui Autonomous Region 750004, P.R. China.
Department of Orthopedics, Ningxia People's Hospital, Yinchuan, Ningxia Hui Autonomous Region 750004, P.R. China.
Exp Ther Med. 2021 Jul;22(1):696. doi: 10.3892/etm.2021.10128. Epub 2021 May 2.
In the present study, a model of glioma stem cells (GSCs) was established and combined with molecular targeting drugs in order to observe its inhibitory effect on the proliferation and biological characteristics of GSCs, with the aim of providing a potential target for the treatment of glioma. On the basis of a relatively classical induction strategy with neuron induction medium, a large number of GSC-like cells in good condition and globular growth were amplified , which had the potential to differentiate into neurons, oligodendrocytes and astrocytes/glioma cells. It was observed that the interference with dynamin-related protein 1 expression using Mdivi-1, a mitochondrial mitotic inhibitor, at the optimal concentration, decreased the expression level of stem cell-associated genes, inhibited proliferation and promoted apoptosis in GSCs. The present study provided an experimental basis for a novel strategy of cancer treatment with tumor stem cells as the target.
在本研究中，建立了胶质瘤干细胞（GSCs）模型并与分子靶向药物相结合，以观察其对GSCs增殖和生物学特性的抑制作用，旨在为胶质瘤治疗提供潜在靶点。在使用神经元诱导培养基的相对经典诱导策略的基础上，扩增出大量状态良好且呈球状生长的GSC样细胞，这些细胞具有分化为神经元、少突胶质细胞和星形胶质细胞/胶质瘤细胞的潜力。观察到，使用线粒体有丝分裂抑制剂Mdivi-1在最佳浓度下干扰动力相关蛋白1的表达，可降低干细胞相关基因的表达水平，抑制GSCs的增殖并促进其凋亡。本研究为以肿瘤干细胞为靶点的癌症治疗新策略提供了实验依据。
