Wan Nianqing, Zheng Jianxiao
Department of Integrated Traditional Chinese and Western Medicine, Yueqing People's Hospital, Yueqing, Zhejiang 325600, P.R. China.
Oncol Lett. 2021 Jul;22(1):507. doi: 10.3892/ol.2021.12768. Epub 2021 May 2.
Numerous studies have shown that the dysregulation of microRNA (miRNA/miR) is an important factor in the pathogenesis of lung cancer. However, the role of miR-891a-5p in non-small cell lung cancer (NSCLC) remains unclear. Therefore, the present study aimed to examine the clinical value and biological function of miR-891a-5p in NSCLC. The mRNA expression level of miR-891a-5p in NSCLC was determined using reverse transcription-quantitative PCR and was used to determine the diagnostic value of miR-891a-5p, by creating a receiver operating characteristic curve. Kaplan-Meier and Cox regression analyses were used to evaluate its prognostic value in patients with NSCLC. Furthermore, cell experiments were performed to investigate the underlying mechanisms and functional role of miR-891a-5p in NSCLC progression. The results indicated that miR-891a-5p expression level was significantly higher in serum and tissues from patients with NSCLC and NSCLC cell lines. In addition, serum miR-891a-5p was found to have a diagnostic value in patients with NSCLC, and the increase in the expression level of miR-891a-5p in tumor tissues was associated with differentiation, and the tumor, node and metastases stages of cancer, which could be used for NSCLC prognosis. In addition, the experiments revealed that NSCLC cell proliferation, invasion and migration were significantly increased by the overexpression of miR-891a-5p and were significantly reduced by its downregulation. Furthermore, a luciferase reporter assay and the protein expression levels of HOXA5 showed that HOXA5 might be a miR-891a-5p target gene. In summary, the results indicated that high miR-891a-5p expression level could be a novel biomarker in patients with NSCLC and that it promoted tumor cell proliferation, invasion and migration. HOXA5 may be a target of miR-891a-5p, which may mediate miR-891a-5p function in NSCLC.
大量研究表明，微小RNA（miRNA/miR）失调是肺癌发病机制中的一个重要因素。然而，miR-891a-5p在非小细胞肺癌（NSCLC）中的作用仍不清楚。因此，本研究旨在探讨miR-891a-5p在NSCLC中的临床价值和生物学功能。采用逆转录定量PCR测定NSCLC中miR-891a-5p的mRNA表达水平，并通过绘制受试者工作特征曲线来确定miR-891a-5p的诊断价值。采用Kaplan-Meier法和Cox回归分析评估其在NSCLC患者中的预后价值。此外，进行细胞实验以研究miR-891a-5p在NSCLC进展中的潜在机制和功能作用。结果表明，NSCLC患者血清和组织以及NSCLC细胞系中miR-891a-5p表达水平显著升高。此外，发现血清miR-891a-5p对NSCLC患者具有诊断价值，肿瘤组织中miR-891a-5p表达水平的升高与肿瘤分化、肿瘤、淋巴结和转移分期相关，可用于NSCLC的预后判断。此外，实验显示，miR-891a-5p过表达显著增加NSCLC细胞的增殖、侵袭和迁移，而下调miR-891a-5p则使其显著降低。此外，荧光素酶报告基因检测和HOXA5蛋白表达水平显示，HOXA5可能是miR-891a-5p的靶基因。综上所述，结果表明高miR-891a-5p表达水平可能是NSCLC患者的一种新型生物标志物，并且它促进肿瘤细胞的增殖、侵袭和迁移。HOXA5可能是miR-891a-5p的靶点，其可能介导miR-891a-5p在NSCLC中的功能。
