Li Bin, Zhang Fengmin, Li Hong
Department of Respiratory Medicine, Shouguang People's Hospital, Shouguang, Shandong 262700, P.R. China.
Department of Obstetrics, Shouguang People's Hospital, Shouguang, Shandong 262700, P.R. China.
Exp Ther Med. 2020 Dec;20(6):172. doi: 10.3892/etm.2020.9302. Epub 2020 Oct 9.
Non-small cell lung cancer (NSCLC) is a malignant tumor, which presents with a high 5-year mortality rate owing to the lack of an effective early screening tool and the absence of obvious early symptoms. MicroRNAs (miRs/miRNAs) have attracted increasing attention due to their significant clinical value in the diagnosis and prognosis of various human malignancies. The present study aimed to investigate the expression levels of microRNA (miR)-1225-5p in NSCLC and to analyze its prognostic value and biological role. The expression levels of miR-1225-5p in the tissues of patients with NSCLC and NSCLC cell lines were analyzed using reverse transcription-quantitative PCR. The association between miR-1225-5p expression levels and the clinicopathological features of patients with NSCLC was analyzed using a χ test. The prognostic value of miR-1225-5p in NSCLC was analyzed using both Kaplan Meier survival and Cox regression analyses, and the effects of miR-1225-5p on NSCLC cell proliferation, migration and invasion were examined. The results revealed that the expression levels of miR-1225-5p were significantly downregulated in NSCLC tissues compared with normal control tissues. Furthermore, miR-1225-5p was discovered to be a potential independent prognostic factor in NSCLC. The inhibition of miR-1225-5p in NSCLC cell lines led to increased cell proliferation, migration and invasion, whereas miR-1225-5p overexpression exerted the opposite effects in these cells. In conclusion, the findings of the present study indicated that the downregulated expression levels of miR-1225-5p in NSCLC may predict a poor prognosis in patients and suggested miR-1225-5p as a potential therapeutic target for NSCLC.
非小细胞肺癌（NSCLC）是一种恶性肿瘤，由于缺乏有效的早期筛查工具且无明显早期症状，其5年死亡率很高。微小RNA（miRs/miRNAs）因其在各种人类恶性肿瘤的诊断和预后方面具有重大临床价值而受到越来越多的关注。本研究旨在调查微小RNA（miR）-1225-5p在NSCLC中的表达水平，并分析其预后价值和生物学作用。采用逆转录定量PCR分析NSCLC患者组织及NSCLC细胞系中miR-1225-5p的表达水平。采用χ检验分析miR-1225-5p表达水平与NSCLC患者临床病理特征之间的关联。采用Kaplan Meier生存分析和Cox回归分析评估miR-1225-5p在NSCLC中的预后价值，并检测miR-1225-5p对NSCLC细胞增殖、迁移和侵袭的影响。结果显示，与正常对照组织相比，NSCLC组织中miR-1225-5p的表达水平显著下调。此外，发现miR-1225-5p是NSCLC中一个潜在的独立预后因素。在NSCLC细胞系中抑制miR-1225-5p会导致细胞增殖、迁移和侵袭增加，而miR-1225-5p过表达则在这些细胞中产生相反的作用。总之，本研究结果表明，NSCLC中miR-1225-5p表达水平下调可能预示患者预后不良，并提示miR-1225-5p作为NSCLC的潜在治疗靶点。
