Irino S, Murakami T, Fujita T
Arch Histol Jpn. 1977 Sep;40(4):297-304. doi: 10.1679/aohc1950.40.297.
Human spleen specimens conductive-stained by the tannin-osmium method were microdissected under the scanning electron microscope and it was revealed that arteries open through one or two end-pores (3-4 mu in diameter) into the reticular cell meshes in the Billroth cord and in the perifollicular area. The endothelial cells lining the arterial terminal portion, which may be swollen into a saccular space, are perforated intracellularly. Dumb-bell shaped erythrocytes are seen passing through these side-pores (1-2 mu in diameter). The termination of arteries not in sinuses but in the cordal reticulum was also confirmed by scanning electron microscopy of blood vascular casts of methacrylate resin. These findings evidence that the "open" theory is valid in the human spleen.
用单宁酸锇法进行传导染色的人脾脏标本在扫描电子显微镜下进行显微解剖，结果显示动脉通过一个或两个端孔（直径3 - 4微米）开口进入脾索和滤泡周围区域的网状细胞网中。动脉末端部分的内皮细胞可能肿胀成囊状间隙，细胞内有穿孔。可见哑铃形红细胞通过这些侧孔（直径1 - 2微米）。通过甲基丙烯酸树脂血管铸型的扫描电子显微镜也证实了动脉不是终止于血窦而是终止于脾索网状组织。这些发现证明“开放”理论在人脾脏中是有效的。
