Dawe R D, Wright J L
National Research Council of Canada, Atlantic Research Laboratory, Halifax, Nova Scotia.
Lipids. 1988 Apr;23(4):355-8.
Current methods for determining the regiochemistry of monoacylglycerols are lengthy, tedious and aggravated by the ready isomerization of 2-acyl-sn-glycerols. A new method employs a very rapid adsorption chromatography step in which isomerization is kept to a minimum, followed by formation of the isopropylidene derivatives using mild procedures. These cyclic derivatives of 1- and 3-acyl-sn-glycerols and 2-acyl-sn-glycerols are stable thermally and display certain characteristic 13C NMR resonances. Integration of these resonances yields directly the proportion of monoacylglycerol isomers in the mixture. The method was applied to the analysis of monoacylglycerols produced by enzymatic hydrolysis of synthetic and naturally occurring triacylglycerols.
目前用于确定单酰甘油区域化学的方法冗长、繁琐，并且由于2-酰基-sn-甘油容易异构化而变得更加复杂。一种新方法采用了非常快速的吸附色谱步骤，其中异构化被保持在最低限度，随后使用温和的程序形成亚异丙基衍生物。这些1-和3-酰基-sn-甘油以及2-酰基-sn-甘油的环状衍生物热稳定性良好，并显示出某些特征性的13C NMR共振。对这些共振进行积分可直接得出混合物中单酰甘油异构体的比例。该方法被应用于分析由合成和天然存在的三酰甘油酶促水解产生的单酰甘油。
