Zhou Quan, Wu Cenhao, Zha Jiali, Ge Jun, Yan Qi, Wang Yingjie, Song Dawei, Zou Jun
Department of Orthopaedic Surgery, The Affiliated Huai'an Hospital of Xuzhou Medical University, Huai'an, Jiangsu 223002, China.
Department of Orthopaedic Surgery, The First Affiliated Hospital of Soochow University, 188 Shizi St, Suzhou, Jiangsu 215006, China.
Open Life Sci. 2020 May 6;15:209-216. doi: 10.1515/biol-2020-0021. eCollection 2020.
While calcium phosphate cement (CPC) is recognized as one of the most likely substitutes for the conventional Polymethylmethacrylate (PMMA), there are very few studies about its intradiscal leakage consequences. Herein, the goal of our study was to examine the effect of CPC particles on the ERK (extracellular regulatory kinase) pathway in human nucleus pulposus cell (HNPC) degeneration. Different concentrations of CPC particles (0.00‰, 0.01‰, 0.05‰, 0.1‰ v/v) were added to human nucleus pulposus cell cultures. After 10 days of treatment, HNPC biological behaviors and degeneration degree were analyzed by CCK-8 assay, crystal violet staining, flow cytometer and western blot. The effect of CPC on the ERK pathway was also analyzed by western blot. After activating the ERK path by overexpressing Ras, HNPCs' biological behaviors and degeneration degree were analyzed again. We found that CPC particles had a negative effect on human nucleus pulposus cells (HNPCs), which are mainly reflected in cell growth and the cell cycle. After activation of the ERK signaling pathway, the negative effects of CPC on cell growth and the cell cycle were significantly reduced and the degeneration degree of HNPCs was reversed. CPC particles can probably block the activation of the ERK pathway, thus causing the HNPCs' degeneration.
虽然磷酸钙骨水泥（CPC）被认为是传统聚甲基丙烯酸甲酯（PMMA）最有可能的替代品之一，但关于其椎间盘内渗漏后果的研究却很少。在此，我们研究的目的是探讨CPC颗粒对人髓核细胞（HNPC）退变中细胞外调节激酶（ERK）通路的影响。将不同浓度的CPC颗粒（0.00‰、0.01‰、0.05‰、0.1‰ v/v）添加到人髓核细胞培养物中。处理10天后，通过CCK-8法、结晶紫染色、流式细胞仪和蛋白质免疫印迹法分析HNPC的生物学行为和退变程度。还通过蛋白质免疫印迹法分析CPC对ERK通路的影响。通过过表达Ras激活ERK通路后，再次分析HNPC的生物学行为和退变程度。我们发现CPC颗粒对人髓核细胞（HNPC）有负面影响，主要体现在细胞生长和细胞周期方面。ERK信号通路激活后，CPC对细胞生长和细胞周期的负面影响显著降低，HNPC的退变程度得到逆转。CPC颗粒可能会阻断ERK通路的激活，从而导致HNPC退变。
