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从牛趾间皮炎中分离出的KS1的高质量基因组序列。

High quality genome sequence of KS1 isolated from bovine digital dermatitis.

作者信息

Espiritu Hector M, Mamuad Lovelia L, Jin Su-Jeong, Kim Seon-Ho, Lee Sang-Suk, Cho Yong-Il

机构信息

Department of Animal Science and Technology, Sunchon National University, Suncheon 57922, Korea.

出版信息

J Anim Sci Technol. 2020 Nov;62(6):948-951. doi: 10.5187/jast.2020.62.6.948. Epub 2020 Nov 30.

Abstract

KS1, a fastidious anaerobe, was isolated from a bovine digital dermatitis (BDD)-infected dairy cattle in Chungnam, Korea. Initial data indicated that KS1 exhibited putative virulent phenotypic characteristics. This study reports the whole genome assembly and annotation of KS1 (KCTC14157BP) to assist in the identification of putative pathogenicity related factors. The whole genome of KS1 was sequenced using PacBio RSII and Illumina HiSeqXTen platforms. The assembled KS1 genome comprises 16 contigs with a total size of 3,769,422 bp and an overall guanine-cytosine (GC) content of 40.03%. Annotation revealed 3,460 protein-coding genes, as well as 49 transfer RNA- and 6 ribosomal RNA-coding genes. The results of this study provide insight into the pathogenicity of KS1.

摘要

KS1是一种苛求厌氧菌,从韩国忠南一头感染牛趾间皮炎(BDD)的奶牛中分离得到。初步数据表明KS1具有假定的毒力表型特征。本研究报告了KS1(KCTC14157BP)的全基因组组装和注释,以协助鉴定假定的致病相关因子。使用PacBio RSII和Illumina HiSeqXTen平台对KS1的全基因组进行了测序。组装后的KS1基因组由16个重叠群组成,总大小为3,769,422 bp,鸟嘌呤-胞嘧啶(GC)总体含量为40.03%。注释显示有3460个蛋白质编码基因,以及49个转运RNA编码基因和6个核糖体RNA编码基因。本研究结果为深入了解KS1的致病性提供了依据。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9b07/7721586/c36d6d265cbb/jast-62-6-948-g1.jpg

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