School of Optometry, The Hong Kong Polytechnic University, Kowloon, Hong Kong.
School of Clinical Medicine, University of Cambridge, Addenbrooke's Hospital, Cambridge, United Kingdom.
Vis Neurosci. 2021 May 14;38:E008. doi: 10.1017/S0952523821000079.
Our previous research showed that increased phosphorylation of connexin (Cx)36 indicated extended coupling of AII amacrine cells (ACs) in the rod-dominant mouse myopic retina. This research will determine whether phosphorylation at serine 276 of Cx35-containing gap junctions increased in the myopic chicken, whose retina is cone-dominant. Refractive errors and ocular biometric dimensions of 7-days-old chickens were determined following 12 h and 7 days induction of myopia by a -10D lens. The expression pattern and size of Cx35-positive plaques were examined in the early (12 h) and compensated stages (7 days) of lens-induced myopia (LIM). At the same time, phosphorylation at serine 276 (functional assay) of Cx35 in strata 5 (S5) of the inner plexiform layer was investigated. The axial length of the 7 days LIM eyes was significantly longer than that of non-LIM controls (P < 0.05). Anti-phospho-Ser276 (Ser276-P)-labeled plaques were significantly increased in LIM retinas at both 12 h and 7 days. The density of Ser276-P of Cx35 was observed to increase after 12 h LIM. In the meanwhile, the areas of existing Cx35 plaques did not change. As there was more phosphorylation of connexin35 at Ser276 at both the early and late stages (12 h) and 7 days of LIM chicken retinal activity, the coupling with ACs could be increased in myopia development of the cone-dominated chicken retina.
我们之前的研究表明，缝隙连接蛋白（Cx）36 的磷酸化增加表明在 rod-dominant 型近视小鼠视网膜中 AII 无长突细胞（AC）的偶联得到了扩展。这项研究将确定在 cone-dominant 型鸡的近视眼中，Cx35 包含的缝隙连接中的丝氨酸 276 的磷酸化是否增加。通过-10D 透镜诱导近视 12 小时和 7 天后，测定 7 日龄鸡的屈光度和眼生物测量尺寸。在 lens-induced myopia（LIM）的早期（12 小时）和补偿阶段（7 天），检查 Cx35 阳性斑块的表达模式和大小。同时，在神经内丛状层的 S5 层研究 Cx35 丝氨酸 276 磷酸化（功能测定）。7 天 LIM 眼的眼轴长度明显长于非 LIM 对照组（P < 0.05）。在 LIM 视网膜中，12 小时和 7 天时，抗磷酸化丝氨酸 276（Ser276-P）标记的斑块明显增加。在 12 小时 LIM 后观察到 Cx35 的 Ser276-P 密度增加。与此同时，现有的 Cx35 斑块的面积没有变化。由于在 LIM 鸡视网膜活动的早期（12 小时）和晚期以及 7 天都有更多的 Cx35 在 Ser276 处磷酸化，因此在 cone-dominant 型鸡视网膜的近视发展过程中，AC 之间的偶联可能会增加。
