Van Der Zee J S, Van Swieten P, Aalberse R C
Central Laboratory of the Netherlands Red Cross Blood Transfusion Service, Amsterdam.
Mol Immunol. 1988 Apr;25(4):345-54. doi: 10.1016/0161-5890(88)90029-6.
The mechanism of activation of the classical pathway of human complement by house-dust extracts and its relevance to atopic disease was studied. Our results confirm that for most sera of adults, house-dust extract is, on a weight basis, a more potent C-activator than aggregated human IgG or lipopolysaccharide (endotoxin). Naturally occurring IgM antibodies directed against ubiquitous polysaccharides appeared to be the dominant factor in the C1 activation by house-dust extracts in human sera. Large variations were found between sera with respect to the concns of these IgM antibodies as measured by C1 activation or fixation of haemolytic complement. The IgM antibody titre was, however, not associated with atopic disease. Consequently, we do not support the hypothesis put forward by Berrens et al. (1978) (Allergol. Immunopath. 6, 45-54) that there might be a relation between atopy and enhanced reactivity of serum complement with allergenic extracts. More than 90% of the C-activating potential of allergen extracts like house dust was found in the fractions with high mol. wt material (mol. wt greater than 100 K). Therefore, these antigens are easily separated from the known IgE-binding major allergens of house-dust mite and cat dander.
研究了屋尘提取物激活人补体经典途径的机制及其与特应性疾病的相关性。我们的结果证实，对于大多数成人血清而言，按重量计算，屋尘提取物是比聚合人IgG或脂多糖（内毒素）更强的补体激活剂。针对普遍存在的多糖的天然存在的IgM抗体似乎是屋尘提取物激活人血清中C1的主要因素。通过C1激活或溶血补体固定来测量，这些IgM抗体的浓度在不同血清之间存在很大差异。然而，IgM抗体滴度与特应性疾病无关。因此，我们不支持Berrens等人（1978年）提出的假说（《变态反应与免疫病理学》6，45 - 54），即特应性与血清补体对变应原提取物的反应性增强之间可能存在关联。发现屋尘等变应原提取物超过90%的补体激活潜能存在于高分子量物质（分子量大于100K）部分。因此，这些抗原很容易与屋尘螨和猫皮屑中已知的IgE结合主要变应原分离。
