Mayekawa MFG Co., Ltd., 2000 Tatsuzawa, Moriya, Ibaraki 302-0118, Japan.
Cryo Letters. 2020 Jul-Aug;41(4):230-236.
Human induced pluripotent stem cells (hiPSCs) are valuable resources for cell therapy and drug discovery. Cryopreservation is a key technique used to realize these applications, which require a large number of cells. However, standard protocols for the preservation of the adherent cells involve multiple complicated steps, which can lead to technical difficulties.
To develop a more efficient method for cryopreservation of adherent cells using culture dishes.
Ice-seeding treatment was employed to avoid intercellular freezing, and rapid warming used to improve cell viability.
The immediate survival rate after thawing was 48%. The recovery period of cells cryopreserved by the dish culture method was shortened upon subsequent passage culture, and the time for re-cultured cells to reach the appropriate confluency was reduced by two days.
The hiPSCs can be successfully cryopreserved in culture dishes with improved viability and faster recovery. The optimization of the ice-seeding temperature and cooling rate increased the survival rate.
人类诱导多能干细胞（hiPSCs）是细胞治疗和药物发现的有价值资源。冷冻保存是实现这些应用的关键技术，需要大量的细胞。然而，用于保存贴壁细胞的标准方案涉及多个复杂步骤，这可能导致技术困难。
开发一种更有效的使用培养皿冷冻保存贴壁细胞的方法。
采用冰种处理避免细胞间结冰，快速升温提高细胞活力。
解冻后的即刻存活率为 48%。通过皿培养法冷冻保存的细胞的恢复周期在随后的传代培养中缩短，再培养细胞达到适当汇合度的时间缩短了两天。
hiPSCs 可以在培养皿中成功冷冻保存，提高了细胞活力，恢复速度更快。优化冰种温度和冷却速率可提高存活率。
