不同渗透保护剂对猫附睾精子质量的影响。
Effect of Different Permeable Cryoprotectants on the Quality of Cat Epididymal Spermatozoa.
机构信息
Molecular Agricultural Biosciences Cluster, Institute of Molecular Biosciences (MB), Mahidol University, Nakhon Pathom 73170, Thailand.
出版信息
Cryo Letters. 2020 Jul-Aug;41(4):237-244.
BACKGROUND
Permeable cryoprotectants (CPAs) are required for successful sperm cryopreservation.
OBJECTIVE
To investigate the efficacy of adding different CPAs to a freezing extender on cat epididymal sperm quality.
METHODS
Epididymal spermatozoa were suspended in Tris-glucose-citrate-egg yolk extender supplemented either with glycerol, methanol, formamide, ethylene glycol (EG), propylene glycol (PG) and dimethylsulfoxide (DMSO), all at 5% (v/v), and then cryopreserved. Sperm motility, viability, functional membrane integrity, morphology and acrosome integrity were examined at post-thaw.
RESULTS
Glycerol, formamide, EG and DMSO exhibited good, comparable cryoprotective effects, whereas PG showed moderate cryoprotection. Sperm viability in PG was lower than that in glycerol and EG, but was not different in formamide and DMSO. Contrarily, the least efficacy was observed in methanol. Interestingly, the CPA type has no effect on functional membrane integrity and morphology.
CONCLUSION
Using Tris-glucose-citrate-egg yolk extender, formamide, EG and DMSO could substitute glycerol as permeable CPAs for cat epididymal sperm cryopreservation.
背景
渗透保护剂（CPAs）是成功进行精子冷冻保存所必需的。
目的
研究在冷冻液中添加不同 CPAs 对猫附睾精子质量的效果。
方法
将附睾精子悬浮在 Tris-葡萄糖-柠檬酸盐-卵黄液中，添加 5%（v/v）的甘油、甲醇、甲酰胺、乙二醇（EG）、丙二醇（PG）和二甲基亚砜（DMSO），然后进行冷冻保存。在解冻后检查精子的运动能力、活力、功能膜完整性、形态和顶体完整性。
结果
甘油、甲酰胺、EG 和 DMSO 表现出良好的、可比的保护效果，而 PG 则表现出中等的保护效果。PG 中的精子活力低于甘油和 EG，但在甲酰胺和 DMSO 中没有差异。相反，甲醇的效果最差。有趣的是，CPA 类型对功能膜完整性和形态没有影响。
结论
使用 Tris-葡萄糖-柠檬酸盐-卵黄液，甲酰胺、EG 和 DMSO 可以替代甘油作为猫附睾精子冷冻保存的渗透 CPAs。
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