Institute of Food Crops, Jiangsu Academy of Agricultural Sciences, Jiangsu, China.
State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou, China.
Plant Signal Behav. 2021 Sep 2;16(9):1926813. doi: 10.1080/15592324.2021.1926813. Epub 2021 May 14.
Ferredoxins (Fds) play a unique and important role in photosynthetic electron transport. Recently, we characterized the function of Fd1 in rice ( L.), showing that Fd1 is the primary photosynthetic electron transport protein and that Fd1 participates in carbon assimilation. However, the subcellular localization and specific functions of other Fds in rice are not yet fully understood. Here, our subcellular localization analysis of the seven Fds in rice showed that they are located in the chloroplasts of photosynthetic tissues and the plastids of non-photosynthetic tissues. Moreover, qRT-PCR indicated that transcript levels were highest in photosynthetic tissues, while transcript levels were highest in non-photosynthetic tissues. Collectively, our results suggest that rice Fds are located in chloroplasts/plastids, but may function in different tissues, and Fd4 may be a non-photosynthetic type Fd.
铁氧还蛋白(Fds)在光合作用电子传递中发挥着独特而重要的作用。最近,我们对水稻中的 Fd1 功能进行了表征,表明 Fd1 是主要的光合作用电子传递蛋白,并且 Fd1 参与碳同化。然而,水稻中其他 Fds 的亚细胞定位和特定功能尚不完全清楚。在这里,我们对水稻中的 7 种 Fds 的亚细胞定位分析表明,它们位于光合作用组织的叶绿体和非光合作用组织的质体中。此外,qRT-PCR 表明在光合作用组织中表达水平最高,而在非光合作用组织中表达水平最高。总的来说,我们的结果表明,水稻 Fds 位于叶绿体/质体中,但可能在不同组织中发挥功能,并且 Fd4 可能是非光合作用类型的 Fd。