Department of Biochemistry, Faculty of Veterinary Medicine, Atatürk University, 25240 Erzurum, Turkey.
Department of Biochemistry, Faculty of Veterinary Medicine, Bingol University, 12000 Bingol, Turkey.
Life Sci. 2021 Jul 15;277:119610. doi: 10.1016/j.lfs.2021.119610. Epub 2021 May 11.
Cadmium (Cd) is a toxic heavy metal that causes severe toxic effects on different tissues including liver and kidney. Therefore the research for alternatives to reduce the damage caused by Cd has substantial importance. This study was performed to examine the possible modulatory effects of carvacrol (CRV) against Cd-induced hepatorenal toxicities and the possible mechanisms underlying these effects.
In the present study, 35 male Wistar rats were randomly divided into 5 groups. The rats were treated with Cd (25 mg/kg) and treated with CRV (25 and 50 mg/kg body weight) for 7 consecutive days.
CRV could modulate Cd-induced elevations of ALT, ALP, AST, urea, creatinine, MDA and enhance antioxidant enzymes' activities such as SOD, CAT, and GPx, and GSH's level. CRV also reversed the changes in levels of inflammatory biomarker and apoptotic genes that include NF-κB, Bcl-3, MAPK-14, iNOS, COX-2, MPO, PGE2, Bax, Bcl-2, P53, Caspase-9, Caspase-6 and Caspase-3 in both tissues. The levels of 8-OHdG in the Cd-induced liver and kidney tissues were modulated after CRV treatment. Furthermore, CRV treatment considerably lowered Cd, Na, Fe, and Zn content while increased K, Ca, Mg and Cu contents in both tissues as compared to the Cd-exposed rats.
The results of the present study revealed that CRV supplementation could be a promising strategy to protect the liver and kidney tissues against Cd-induced oxidative damage, inflammation and apoptosis.
镉（Cd）是一种有毒重金属，对包括肝脏和肾脏在内的不同组织造成严重的毒性作用。因此，寻找替代品以减少 Cd 造成的损害具有重要意义。本研究旨在研究香芹酚（CRV）对 Cd 诱导的肝肾功能毒性的可能调节作用及其潜在机制。
本研究中，将 35 只雄性 Wistar 大鼠随机分为 5 组。大鼠连续 7 天用 Cd（25mg/kg）处理，并分别用 CRV（25 和 50mg/kg 体重）处理。
CRV 可调节 Cd 诱导的 ALT、ALP、AST、尿素、肌酐、MDA 升高，并增强 SOD、CAT 和 GPx 等抗氧化酶的活性，以及 GSH 水平。CRV 还逆转了 NF-κB、Bcl-3、MAPK-14、iNOS、COX-2、MPO、PGE2、Bax、Bcl-2、P53、Caspase-9、Caspase-6 和 Caspase-3 等炎症生物标志物和凋亡基因在两种组织中的变化。CRV 处理还调节了 Cd 诱导的肝和肾组织中 8-OHdG 的水平。此外，与 Cd 暴露大鼠相比，CRV 处理显著降低了 Cd、Na、Fe 和 Zn 含量，同时增加了 K、Ca、Mg 和 Cu 含量。
本研究结果表明，CRV 补充可能是一种有前途的策略，可保护肝脏和肾脏组织免受 Cd 诱导的氧化损伤、炎症和凋亡。
