Department of Chemical Engineering, School of Chemical and Petroleum Engineering, Shiraz University, Shiraz 71348-51154, Iran.
Department of Chemical Engineering, School of Chemical and Petroleum Engineering, Shiraz University, Shiraz 71348-51154, Iran.
Int J Pharm. 2021 Jun 15;603:120698. doi: 10.1016/j.ijpharm.2021.120698. Epub 2021 May 12.
Treatment of skin injuries is still facing major challenges, such as chronicity and infections, particularly those caused by multi-drug resistance pathogens. An effective treatment of such wounds should accelerate the wound healing process while preventing bacterial contamination. Here, a novel core-shell nanofiber mat was fabricated comprising gelatin/polyvinyl alcohol (as a core) and aloe vera/arabinose/polyvinylpyrrolidone (as a shell) for accelerating the healing process of bacteria-infected wounds. Trachyspermum Ammi (Ajwain) essential oil (EO), as a potent and natural antimicrobial agent against microorganisms, was incorporated into the core of nanofiber mats using coaxial electrospinning. The microscopy images demonstrated the successful fabrication of the core-shell structure with a uniform fiber size of 564 ± 106.35 nm. Moreover, Ajwain EO-loaded nanofiber mat (core-shell/EO) provided excellent antimicrobial activity and antioxidant ability. The in vitro and ex vivo release of Ajwain EO from the fabricated nanofiber mat corroborated a prolonged release profile. Furthermore, in vivo antibacterial activity, wound closure, and histomorphological examinations showed the high efficacy of the core-shell/EO mat in the treatment of Staphylococcus aureus-infected full-thickness rat wounds compared to standard control treatment with a gauze. Overall, these results represent the core-shell/EO mat's potential as a newly developed wound dressing for bacteria-infected full-thickness skin injuries.
皮肤损伤的治疗仍然面临着重大挑战，如慢性和感染，特别是由多药耐药病原体引起的感染。这种伤口的有效治疗方法应该加速伤口愈合过程，同时防止细菌污染。在这里，我们制备了一种新型的核壳纳米纤维垫，由明胶/聚乙烯醇（作为核）和芦荟/阿拉伯糖/聚乙烯吡咯烷酮（作为壳）组成，用于加速细菌感染伤口的愈合过程。荜澄茄精油（EO）作为一种对抗微生物的有效且天然的抗菌剂，被同轴静电纺丝法纳入纳米纤维垫的核中。显微镜图像显示成功地制备了具有均匀纤维尺寸为 564 ± 106.35nm 的核壳结构。此外，荜澄茄精油负载的纳米纤维垫（核壳/EO）具有优异的抗菌活性和抗氧化能力。从所制备的纳米纤维垫中释放的荜澄茄精油的体外和体内释放证实了其具有延长的释放特性。此外，体内抗菌活性、伤口闭合和组织形态学检查表明，与标准的纱布对照治疗相比，核壳/EO 垫在治疗金黄色葡萄球菌感染的全层大鼠伤口方面具有很高的疗效。总的来说，这些结果表明核壳/EO 垫作为一种新开发的用于治疗全层皮肤细菌感染的伤口敷料具有潜力。
