Xue Qiannan, Kan Xinyuan, Pan Zhihao, Li Zheyu, Pan Wenwei, Zhou Feng, Duan Xuexin
State Key Laboratory of Precision Measuring Technology & Instruments, School of Precision Instruments and Optoelec-tronics Engineering, Tianjin University, Tianjin, 300072, China.
State Key Laboratory of Precision Measuring Technology & Instruments, School of Precision Instruments and Optoelec-tronics Engineering, Tianjin University, Tianjin, 300072, China.
Biosens Bioelectron. 2021 Aug 15;186:113286. doi: 10.1016/j.bios.2021.113286. Epub 2021 May 3.
The current ongoing outbreak of Coronavirus Disease 2019 (COVID-19) has globally affected the lives of more than one hundred million people. RT-PCR based molecular test is recommended as the gold standard method for diagnosing current infections. However, transportation and processing of the clinical sample for detecting virus require an expert operator and long processing time. Testing device enables on-site virus detection could reduce the sample-to-answer time, which plays a central role in containing the pandemic. In this work, we proposed an intelligent face mask, where a flexible immunosensor based on high density conductive nanowire array, a miniaturized impedance circuit, and wireless communication units were embedded. The sub-100 nm size and the gap between the neighbored nanowires facilitate the locking of nanoscale virus particles by the nanowire arrays and greatly improve the detection efficiency. Such a point-of-care (POC) system was demonstrated for coronavirus 'spike' protein and whole virus aerosol detection in simulated human breath. Detection of viral concentration as low as 7 pfu/mL from the atomized sample of coronavirus aerosol mimic was achieved in only 5 min. The POC systems can be readily applied for preliminary screening of coronavirus infections on-site and may help to understand the COVID-19 progression while a patient is under prescribed therapy.
当前正在爆发的2019冠状病毒病（COVID-19）已在全球范围内影响了超过1亿人的生活。基于逆转录聚合酶链反应（RT-PCR）的分子检测被推荐为诊断当前感染的金标准方法。然而，用于检测病毒的临床样本的运输和处理需要专业操作人员且处理时间较长。能够进行现场病毒检测的测试设备可以缩短从样本到得出结果的时间，这在遏制疫情方面起着核心作用。在这项工作中，我们提出了一种智能口罩，其中嵌入了基于高密度导电纳米线阵列的柔性免疫传感器、小型化阻抗电路和无线通信单元。亚100纳米的尺寸以及相邻纳米线之间的间隙有助于纳米线阵列锁定纳米级病毒颗粒，并大大提高检测效率。这种即时检测（POC）系统已被证明可用于在模拟人类呼出气体中检测冠状病毒的“刺突”蛋白和全病毒气溶胶。仅在5分钟内就实现了从模拟冠状病毒气溶胶雾化样本中检测低至7个空斑形成单位/毫升的病毒浓度。该POC系统可轻松用于现场对冠状病毒感染的初步筛查，并可能有助于了解患者在规定治疗期间的COVID-19病情进展。
