Su Yi, Bian Sumin, Pan Dingyi, Xu Yankun, Rong Guoguang, Zhang Hongyong, Sawan Mohamad
College of Biomedical Engineering & Instrument Science, Zhejiang University, Hangzhou 310013, China.
CenBRAIN Neurotech, School of Engineering, Westlake University, Hangzhou 310030, China.
Biosensors (Basel). 2023 Aug 30;13(9):858. doi: 10.3390/bios13090858.
The effective control of infectious diseases, including Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) infection, depends on the availability of rapid and accurate monitoring techniques. However, conventional SARS-CoV-2 detection technologies do not support continuous self-detection and may lead to cross-infection when utilized in medical institutions. In this study, we introduce a prototype of a mask biosensor designed for the long-term collection and self-detection of SARS-CoV-2. The biosensor utilizes the average resonance Rayleigh scattering intensity of Au nanocluster-aptamers. The inter-mask surface serves as a medium for the long-term collection and concentration enhancement of SARS-CoV-2, while the heterogeneous-nucleation nanoclusters (NCs) contribute to the exceptional stability of Au NCs for up to 48 h, facilitated by the adhesion of Ti NCs. Additionally, the biosensors based on Au NC-aptamers exhibited high sensitivity for up to 1 h. Moreover, through the implementation of a support vector machine classifier, a significant number of point signals can be collected and differentiated, leading to improved biosensor accuracy. These biosensors offer a complementary wearable device-based method for diagnosing SARS-CoV-2, with a limit of detection of 10 copies. Given their flexibility, the proposed biosensors possess tremendous potential for the continuous collection and sensitive self-detection of SARS-CoV-2 variants and other infectious pathogens.
包括严重急性呼吸综合征冠状病毒2（SARS-CoV-2）感染在内的传染病的有效控制，取决于快速准确监测技术的可用性。然而，传统的SARS-CoV-2检测技术不支持连续自我检测，在医疗机构使用时可能导致交叉感染。在本研究中，我们介绍了一种用于SARS-CoV-2长期收集和自我检测的口罩生物传感器原型。该生物传感器利用金纳米簇适配体的平均共振瑞利散射强度。口罩表面作为SARS-CoV-2长期收集和浓度增强的介质，而异质成核纳米簇（NCs）通过钛纳米簇的粘附作用，使金纳米簇在长达48小时内具有出色的稳定性。此外，基于金纳米簇适配体的生物传感器在长达1小时内表现出高灵敏度。此外，通过实施支持向量机分类器，可以收集和区分大量点信号，从而提高生物传感器的准确性。这些生物传感器为诊断SARS-CoV-2提供了一种基于可穿戴设备的补充方法，检测限为10个拷贝。鉴于其灵活性，所提出的生物传感器在连续收集和灵敏自我检测SARS-CoV-2变体及其他传染性病原体方面具有巨大潜力。
