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核心结合因子β在腓骨骨折愈合过程中的成骨细胞分化中是必需的。

Core-binding factor beta is required for osteoblast differentiation during fibula fracture healing.

机构信息

The Second Department of Orthopedics, Xianyang Central Hospital, Xianyang, 712000, People's Republic of China.

The Pharmacy Department, Xianyang Central Hospital, Xianyang, 712000, People's Republic of China.

出版信息

J Orthop Surg Res. 2021 May 14;16(1):313. doi: 10.1186/s13018-021-02410-9.

DOI:10.1186/s13018-021-02410-9
PMID:33990210
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8120848/
Abstract

BACKGROUND

Growing evidence has implicated core-binding factor beta (Cbfb) as a contributor to osteoblast differentiation, which plays a key role in fracture healing. Herein, we aimed to assess whether Cbfb affects osteoblast differentiation after fibula fracture.

METHODS

Initially, we established a Cbfb conditional knockout mouse model for subsequent studies. Immunohistochemical staining was conducted to detect the expression of proliferating cell nuclear antigen (PCNA) and collagen II in the fracture end. Next, we isolated and cultured osteoblasts from specific Cbfb conditional knockout mice for BrdU analysis, alkaline phosphatase (ALP) staining, and von Kossa staining to detect osteoblast viability, differentiation, and mineralization, respectively. Western blot analysis and reverse transcription-quantitative polymerase chain reaction (RT-qPCR) were used to detect the expression of osteoblast differentiation-related genes.

RESULTS

The Cbfb conditional knockout mice exhibited downregulated expression of PCNA and collagen II, reduced ALP activity, and mineralization, as well as diminished expression of osteoblast differentiation-related genes. Further, Cbfb knockout exerted no obvious effects on osteoblast proliferation.

CONCLUSIONS

Overall, these results substantiated that Cbfb could promote fibula fracture healing and osteoblast differentiation and thus provided a promising therapeutic target for clinical treatment of fibula fracture.

摘要

背景

越来越多的证据表明核心结合因子β（Cbfb）是成骨细胞分化的贡献者，成骨细胞分化在骨折愈合中起着关键作用。在此，我们旨在评估 Cbfb 是否会影响腓骨骨折后的成骨细胞分化。

方法

首先，我们建立了 Cbfb 条件性敲除小鼠模型，以便进行后续研究。免疫组织化学染色检测增殖细胞核抗原（PCNA）和胶原 II 在骨折端的表达。接下来，我们从特定的 Cbfb 条件性敲除小鼠中分离和培养成骨细胞，进行 BrdU 分析、碱性磷酸酶（ALP）染色和 von Kossa 染色，以分别检测成骨细胞活力、分化和矿化。Western blot 分析和逆转录定量聚合酶链反应（RT-qPCR）用于检测成骨细胞分化相关基因的表达。

结果

Cbfb 条件性敲除小鼠表现出 PCNA 和胶原 II 的表达下调、ALP 活性和矿化减少，以及成骨细胞分化相关基因的表达降低。此外，Cbfb 敲除对成骨细胞增殖没有明显影响。

结论

综上所述，这些结果证实 Cbfb 可以促进腓骨骨折愈合和成骨细胞分化，因此为临床治疗腓骨骨折提供了一个有前途的治疗靶点。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9b76/8120848/dbcc8292eb05/13018_2021_2410_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9b76/8120848/12b27f8d2a2b/13018_2021_2410_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9b76/8120848/aeeb1d309dde/13018_2021_2410_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9b76/8120848/1204c7c33d2f/13018_2021_2410_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9b76/8120848/bbe9f8676c90/13018_2021_2410_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9b76/8120848/a0944a84e891/13018_2021_2410_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9b76/8120848/dbcc8292eb05/13018_2021_2410_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9b76/8120848/12b27f8d2a2b/13018_2021_2410_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9b76/8120848/aeeb1d309dde/13018_2021_2410_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9b76/8120848/1204c7c33d2f/13018_2021_2410_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9b76/8120848/bbe9f8676c90/13018_2021_2410_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9b76/8120848/a0944a84e891/13018_2021_2410_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9b76/8120848/dbcc8292eb05/13018_2021_2410_Fig6_HTML.jpg

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