Plant Biotechnology Laboratory, Post-Graduate Department of Botany, Utkal University, VaniVihar, Bhubaneswar, Odisha, 751004, India.
Sci Rep. 2021 May 14;11(1):10345. doi: 10.1038/s41598-021-89470-3.
Arbitrary (65 RAPD, 25 ISSR, 23 DAMD), gene-targeted (22 SCoT, 33 CBDP) and co-dominant sequence specific (40 SSR) markers were used individually, or in combinations, to examine the genetic variability within and among 70 selected Indian mango genotypes based on geographic origin (East India, West India, North India, South India) and fruit status (Selection, Hybrid, Landrace). The highest genetic variability was demonstrated by the East Indian populations, followed by those from South India, West India, and North India, when measured in terms of Na, Ne, H, I, PB%, Ht and Hs. Interestingly, the local genotypes of Odisha, which forms a part of East Indian populations, showed the highest diversity compared to hybrid or selection groups, suggesting that the indigenous genotypes hold a greater potential for exploiting the unique and favourable alleles. The maximum genetic variability was detected in geographical/fruit status populations with SSRs (Na-1.76/1.88, Ne-1.48/1.51, H-0.28/0.30, I-0.41/0.45, PB%-76.1/86.9, Ht-0.31/0.32 and Hs-0.28/0.30), followed by CBDPs and SCoTs, reflecting their preeminence for examining the level of genetic polymorphism and diversity. Outcome of AMOVA based analyses as well as low-to-moderate coefficient of genetic differentiation (Gst) and high gene flow (Nm) indicated a greater amount of intra-population genetic variation compared to heterogeneity at inter-population level. Information generated through this investigation could facilitate conservation and further exploitation of mango germplasm including genetic improvement through breeding.
随机（65 个 RAPD、25 个 ISSR、23 个 DAMD）、基因靶向（22 个 SCoT、33 个 CBDP）和共显性序列特异性（40 个 SSR）标记物分别或组合使用，用于检查基于地理起源（东印度、西印度、北印度、南印度）和果实状态（选择、杂交、地方品种）的 70 个印度芒果基因型内和基因型间的遗传变异性。从 Na、Ne、H、I、PB%、Ht 和 Hs 等方面衡量，东印度群体表现出最高的遗传变异性，其次是南印度、西印度和北印度群体。有趣的是，奥里萨邦的本地基因型与杂交或选择群体相比表现出最高的多样性，这表明本土基因型具有更大的潜力来利用独特和有利的等位基因。在具有 SSRs 的地理/果实状态群体中检测到最大的遗传变异性（Na-1.76/1.88、Ne-1.48/1.51、H-0.28/0.30、I-0.41/0.45、PB%-76.1/86.9、Ht-0.31/0.32 和 Hs-0.28/0.30），其次是 CBDP 和 SCoT，反映了它们在检查遗传多态性和多样性水平方面的优势。AMOVA 分析的结果以及遗传分化系数（Gst）和基因流（Nm）的低到中等表明，与种群间水平的异质性相比，种群内的遗传变异更大。通过这项研究产生的信息可以促进芒果种质资源的保护和进一步利用，包括通过育种进行遗传改良。
