Research Institution of Subtropical Forestry, Chinese Academy of Forestry, 73, Daqiao Road, Fuyang 311400, Zhejiang, China.
Int J Mol Sci. 2013 Oct 14;14(10):20399-413. doi: 10.3390/ijms141020399.
Calanthe tsoongiana is a rare terrestrial orchid endemic to China, and this species has experienced severe habitat loss and fragmentation. Inter-simple sequence repeat (ISSR) markers were employed to assess the genetic diversity and differentiation of six populations of C. tsoongiana. Based on 124 discernible fragments yielded by eleven selected primers, high genetic diversity was revealed at the species level; however, genetic diversity at the population level was relatively low. High-level genetic differentiation among populations was detected based on analysis of molecular variance (AMOVA), indicating potential limited gene flow. No significant relationship was observed between genetic and geographic distances among the sampled populations. These results suggested that restricted gene flow might be due to habitat fragmentation and reduced population size as a result of human activities. Based on the findings, several conservation strategies were proposed for the preservation of this threatened species.
金线兰是中国特有的一种珍稀陆生兰，该物种生境丧失和片段化较为严重。本研究采用 ISSR 标记对 6 个金线兰居群的遗传多样性和分化进行了评估。基于 11 条随机引物扩增出的 124 条清晰条带，在物种水平上揭示了较高的遗传多样性；然而，居群水平的遗传多样性相对较低。基于 AMOVA 分析，检测到种群间存在高水平的遗传分化，表明潜在的有限基因流。在抽样居群间，遗传距离与地理距离之间没有观察到显著的相关性。这些结果表明，限制基因流可能是由于生境片段化和人类活动导致的种群规模减小所致。基于研究结果，提出了一些保护策略，以保护这一受到威胁的物种。
