Shaygannia E, Ghandehari-Alavijeh R, Tavalaee M, Nasr-Esfahani M H
Department of Animal Biotechnology, Reproductive Biomedicine Research Center, Royan Institute for Biotechnology, ACECR, Isfahan, Iran.
Department of Animal Biotechnology, Reproductive Biomedicine Research Center, Royan Institute for Biotechnology, ACECR; Isfahan Fertility and Infertility Center, Isfahan, Iran.
Cryo Letters. 2020 Nov-Dec;41(6):344-350.
Sperm cryopreservation is presently used for conservation of male gametes in assisted reproduction technologies (ART). Despite the benefits of sperm banking, freeze-thawing process is injurious to sperm integrity due to induced oxidative stress by cold stress. Oxidative stress reduces sperm motility, viability and DNA integrity.
To investigate the effect of alpha lipoic acid (ALA) on human sperm function during the freeze-thawing process.
Thirty semen samples were collected and different concentrations (0, 0.05, 0.1, 0.2, 0.4, 0.8, and 8mM) of ALA were added to a sperm freeze medium and its effects on sperm motility, DNA damage, and lipid peroxidation of frozen-thawed spermatozoa were assessed.
The addition of 0.2 mM ALA to the sperm freeze medium resulted in significant improvement in percentage of sperm motility, less DNA damage and decreased lipid peroxidation during freeze-thawing process (p<0.05).
ALA improves the cryo-protective capacity of sperm freeze medium used for human semen by protecting the sperm from ROS attack induced by the freezing-thawing process. We suggest that sperm freeze medium supplemented with 0.2 mM ALA would be beneficial for the cryopreservation of male gametes in ART.
目前，精子冷冻保存用于辅助生殖技术（ART）中雄性配子的保存。尽管精子库有诸多益处，但冻融过程会因冷应激诱导氧化应激而损害精子完整性。氧化应激会降低精子活力、存活率和DNA完整性。
研究α硫辛酸（ALA）在冻融过程中对人类精子功能的影响。
收集30份精液样本，在精子冷冻培养基中添加不同浓度（0、0.05、0.1、0.2、0.4、0.8和8mM）的ALA，评估其对冻融后精子活力、DNA损伤和脂质过氧化的影响。
在精子冷冻培养基中添加0.2mM ALA可显著提高冻融过程中精子活力百分比，减少DNA损伤并降低脂质过氧化（p<0.05）。
ALA通过保护精子免受冻融过程诱导的活性氧攻击，提高了用于人类精液的精子冷冻培养基的冷冻保护能力。我们建议补充0.2mM ALA的精子冷冻培养基对ART中雄性配子的冷冻保存有益。
