Department of Laboratory Medicine, Karolinska Institutet, Stockholm, Sweden.
Karolinska University Laboratory, Karolinska University Hospital, Stockholm, Sweden.
Int J Cancer. 2021 Sep 15;149(6):1341-1347. doi: 10.1002/ijc.33683. Epub 2021 May 22.
Nonmelanoma skin cancer (NMSC) has a greatly increased incidence among the immunosuppressed and the DNA of human papillomavirus (HPV) is commonly found in these tumors. To investigate if there are any actively transcribed HPV infections in these tumors, we identified all skin cancers diagnosed after solid organ transplantation in Sweden during 1964-2011 (n = 7614 NMSCs) and requested the diagnostic tumor blocks from the corresponding pathology archives. For the present study, we selected diagnostic specimens from 345 NMSC and performed whole genome transcriptome analysis using NovaSeq (Illumina), in comparison with three cervical cancers. Although we obtained an abundance of high-quality paired reads per sample (median of 35 million reads), only 15 NMSC specimens contained HPV transcription. Three specimens had transcription of oncogenic anogenital HPVs (HPV16 and 56), six tumors had transcription of HPVs from the beta-2 species (three HPV38, two with HPV23 and one with HPV107) and then there was one observation each of transcription of HPVs 3, 26, 57, 147, 158, 168 and of two nonestablished HPV types belonging to the gamma genus. In conclusion, transcription of specific HPV types can be found in NMSC among the immunosuppressed, but this is not common.
非黑色素瘤皮肤癌（NMSC）在免疫抑制人群中的发病率大大增加，人类乳头瘤病毒（HPV）的 DNA 通常存在于这些肿瘤中。为了研究这些肿瘤中是否存在活跃转录的 HPV 感染，我们确定了 1964 年至 2011 年间在瑞典进行实体器官移植后诊断出的所有皮肤癌（n=7614 例 NMSC），并从相应的病理档案中请求了诊断性肿瘤块。在本研究中，我们从 345 例 NMSC 中选择了诊断标本，并使用 NovaSeq（Illumina）进行全基因组转录组分析，与 3 例宫颈癌进行比较。尽管我们每个样本获得了大量高质量的配对读取（中位数为 3500 万读取），但只有 15 例 NMSC 标本含有 HPV 转录。三个标本有致癌肛门生殖器 HPV（HPV16 和 56）的转录，六个肿瘤有β-2 种 HPV（HPV38 三种，HPV23 两种，HPV107 一种）的转录，然后还有 HPV3、26、57、147、158、168 的转录各一例，以及两种非已建立的属于γ属的 HPV 类型的转录。总之，在免疫抑制人群的 NMSC 中可以发现特定 HPV 类型的转录，但并不常见。
