School of Life Science, Liaoning Normal University, Dalian, China.
Lamprey Research Center, Liaoning Normal University, Dalian, China.
Curr Microbiol. 2021 Jul;78(7):2631-2639. doi: 10.1007/s00284-021-02530-9. Epub 2021 May 15.
Lamprey, one of the most basal jawless vertebrate, is an excellent model for studying vertebrate evolution, embryo development, and the origin of adaptive immunity. This study investigated the differentially expressed proteins in lamprey leukocytes in response to the co-stimulation of intestinal Aeromonas and Shewanella by using quantitative proteomics techniques. Significant differentially expressed proteins were identified. Gene Ontology annotation and the Kyoto Encyclopedia of Genes and Genomes pathway based on the significant differentially expressed proteins were analyzed. Most of the differentially expressed proteins were predicted to be involved in important signaling pathways. Quantitative real-time polymerase chain reaction was used to verify the expression of differentially expressed proteins at the mRNA level. The expression of some differentially expressed proteins was not consistent at the mRNA and protein levels. Differentially expressed proteins that are essential for lamprey-intestinal bacteria interaction should be identified to understand the lamprey adaptive immune response induced by gut microbiota.
七鳃鳗，一种最原始的无颌脊椎动物，是研究脊椎动物进化、胚胎发育和适应性免疫起源的极好模型。本研究采用定量蛋白质组学技术，研究了七鳃鳗白细胞对肠道气单胞菌和希瓦氏菌共刺激的反应中差异表达蛋白。鉴定到显著差异表达蛋白。基于显著差异表达蛋白的基因本体注释和京都基因与基因组百科全书通路分析。大多数差异表达蛋白预测参与重要的信号通路。采用实时定量聚合酶链反应验证差异表达蛋白在 mRNA 水平的表达。一些差异表达蛋白在 mRNA 和蛋白水平的表达不一致。为了了解肠道微生物群诱导的七鳃鳗适应性免疫反应，应该鉴定对七鳃鳗-肠道细菌相互作用至关重要的差异表达蛋白。
